Signaling Pathways in Vasopressin V2 Receptor-Mediated Regulation of Aquaporin-2
Brief methods: Rat IMCD suspensions were incubated with a vasopressin V2 receptor analog, dDAVP (1 nM), or vehicle for 30 minutes (3 independent pairs). Proteins were digested with trypsin/Lys-C. The peptide samples were labeled with 6-plex TMT and combined. Samples were subjected to phosphopeptide enrichment, using Fe-NTA and TiO2 columns, followed by high pH reversed-phase peptide fractionation. Samples were analyzed by LC-MS/MS (Orbitrap Fusion).
Click on the responses to Vasopressin to open the associated data.
