National Heart, Lung, and Blood Institute

Epithelial Systems Biology Laboratory (ESBL)


AQP2 Interacting Proteins in Rat Inner Medullary Collecting Duct

This database reports proteins identified by mass spectrometry (LC-MS/MS) that bound to water channel protein AQP2 in rat inner medullary collecting duct. IMCD suspenions were treated with vehicle or vasopressin analog (dDAVP, 1 nM) for 15 minutes, followed by in-cell crosslinking using BSOCOES. After incubations, IMCD were harvested by brief centrifugation. The samples were homogenized and solubilized with RIPA. The detergent solubilized fractions were subjected to immunoprecipitation (IP) using AQP2 antibody-coated Dynabeads or preimmune IgG-coated beads as control. The IP products were eluted in Laemmli buffer and loaded for SDS-PAGE. Gel pieces with molecular weight above glycosylated band of AQP2 were cut and prepared for mass spectrometry (LC-MS/MS) analysis according to standard in-gel digestion procedures. Proteins that were found in all three experiments and were statistically significant in either control or dDAVP-treated group were included in the interactome list.

Definitions: R, ratio (peptide intensity in AQP2 antibody specific immunoprecipitation sample)/(peptide intensity in preimmune-IgG immunoprecipitation sample) for each peptide found. INF, infinite value. p-value, P value of student t-test vs log2R=0. -log (P) greater than 1.30 was considered statistically significant. S, significant, P value was not available. NS, not significant. -, not available.

Reference: Chou CL, Hwang G, Hageman DJ, Han L, Agrawal P, Pisitkun T, Knepper MA. Identification of UT-A1- and AQP2-interacting proteins in rat inner medullary collecting duct. Am J Physiol Cell Physiol. 2018;314: C99-C117. PMID: 29046292. Contact: This website was created by Chung-Lin Chou and Mark Knepper. Please address questions or comments to: knep@helix.nih.gov

Database created: September 26, 2016 (download data)

Sort the database by category:

Vehicle
dDAVP
Gene Symbol
Ref-Seq Number
Protein Name
Log2R, Mean±SE
# Peptide
-Log(p-value)
# Exp
Log2R, Mean±SE
# Peptide
-Log(p-value)
# Exp
Aco2NP_077374aconitate hydratase, mitochondrial precursor
2.9±0.6
6
3.25
4
2.1±1.0
5
NS
4
Actn1NP_112267alpha-actinin-1
2.1±0.9
10
1.45
4
3.6±0.6
9
4.48
4
Actn4NP_113863alpha-actinin-4
3.0±0.3
18
9.19
4
2.9±0.3
15
11.14
4
AhnakNP_001178880neuroblast differentiation-associated protein AHNAK
3.3±0.5
14
6.51
4
3.4±0.6
14
4.57
4
Akr1b1NP_036630aldose reductase
3.5±0.5
6
4.16
4
2.2±0.5
8
3.2
4
Aldh1a3NP_695212aldehyde dehydrogenase family 1 member A3
2.4±0.6
10
2.85
4
3.7±0.6
9
4.47
3
Anxa1NP_037036annexin A1
5.1±0.4
13
9.68
4
5.4±0.3
15
14.5
4
Anxa2NP_063970annexin A2
5.2±0.3
22
20.24
4
4.8±0.3
21
19.28
4
Anxa4NP_077069annexin A4
5.2±0.8
8
4.44
4
4.4±0.6
10
5.36
4
Anxa5NP_037264annexin A5
5.2±0.8
8
4.73
4
5.0±0.6
8
6.19
4
Anxa7NP_569100annexin A7
2.8±0.8
3
1.53
2
3.9±0.3
4
4.01
4
Ap1b1NP_058973AP-1 complex subunit beta-1
4.8±0.4
17
10.09
4
4.9±0.5
18
9.76
3
Ap1m2NP_001102466AP-1 complex subunit mu-2
4.5±0.8
5
3.04
4
2.6±2.1
3
NS
3
Ap2b1NP_542150AP-2 complex subunit beta
4.1±0.6
11
5.28
4
5.9±0.4
14
12.19
3
Aqp2NP_037041aquaporin-2
5.3±0.6
19
9.18
4
5.9±0.4
22
15.93
4
Arf5NP_077063ADP-ribosylation factor 5
2.6±1.0
5
1.45
4
3.8±0.8
6
3.15
4
Arg2NP_062041arginase-2, mitochondrial precursor
4.6
2
2.93
4
4.1
1
4.1
4
Atp1a1NP_036636sodium/potassium-transporting ATPase subunit alpha-1 precursor
6.0±0.3
43
29.93
4
6.5±0.2
50
43.74
4
Atp1b1NP_037245sodium/potassium-transporting ATPase subunit beta-1
5.6±0.7
6
4.79
4
5.4±0.5
8
7.61
4
Atp5a1NP_075581ATP synthase subunit alpha, mitochondrial precursor
4.3±0.3
23
17.81
4
4.2±0.3
26
17.05
4
Atp5bNP_599191ATP synthase subunit beta, mitochondrial precursor
3.4±0.5
17
7.39
4
2.4±0.2
17
10.55
4
Atp6v0a1NP_113792V-type proton ATPase 116 kDa subunit a isoform 1
2.3±1.6
6
NS
3
4.2±0.0
6
16.18
4
Atp8a1XP_008768387probable phospholipid-transporting ATPase IA isoform X7
3.9
2
2.9
4
6.1
2
5.88
4
Atp9aXP_008760832probable phospholipid-transporting ATPase IIA
5.4±0.2
7
9.71
4
4.4±0.7
7
4.05
4
BcamNP_113940basal cell adhesion molecule precursor
3.7±0.7
8
3.92
4
5.7±0.4
8
7.35
4
BspryNP_071597B box and SPRY domain-containing protein
3.7±0.6
5
3.18
3
4.4±1.4
4
1.63
4
C3NP_058690complement C3 precursor
6.6
1
S
4
5.0
2
S
4
CanxNP_742005calnexin precursor
5.2±0.4
17
12.09
4
4.7±0.4
14
9.56
4
CapgNP_001013104macrophage-capping protein
2.8±0.7
5
2.29
4
1.4±0.5
5
1.71
4
Capn1NP_062025calpain-1 catalytic subunit
4.1±1.0
6
2.57
4
4.2±0.5
6
4.43
4
Cct4NP_877966T-complex protein 1 subunit delta
4.2±1.5
4
1.47
4
2.1±0.1
2
-
3
Cct5NP_001004078T-complex protein 1 subunit epsilon
2.9
2
2.28
4
2.5
2
-
3
Cdh16NP_001012055cadherin-16 precursor
6.4±1.0
8
4.27
4
5.9±0.9
9
4.93
4
Cds2NP_446095phosphatidate cytidylyltransferase 2
3.6
1
-
3
5.4
2
2.27
4
CltcNP_062172clathrin heavy chain 1
4.2±0.3
39
21.88
4
4.1±0.3
28
18.99
4
Ctnna1NP_001007146catenin alpha-1
5.1±0.2
6
6.8
4
5.5±0.3
5
6.28
4
CttnNP_068640cortactin isoform B
4.9±0.7
5
3.86
4
4.0±0.6
6
4.3
3
Cyfip1NP_001100987cytoplasmic FMR1 interacting protein 1
5.0±1.1
7
3.1
4
5.4±0.8
3
2.61
3
Dlg1NP_036920disks large homolog 1
3.4±0.7
6
2.94
3
4.4±0.7
5
3.26
4
Dync1h1NP_062099cytoplasmic dynein 1 heavy chain 1
4.7±0.3
66
32.29
4
4.1±0.3
69
25.73
4
Dync1i2NP_001257554cytoplasmic dynein 1 intermediate chain 2 isoform 3
4.6±0.6
8
5.12
4
4.8±0.5
8
6.51
4
Eef1a1NP_787032elongation factor 1-alpha 1
4.0±0.2
9
11.69
4
3.1±0.3
11
9.14
4
Eif3aNP_001040552eukaryotic translation initiation factor 3 subunit A
5.2±0.6
15
7.89
4
4.1±0.4
19
8.89
4
Eif3bNP_001026810eukaryotic translation initiation factor 3 subunit B
4.9±0.7
12
6.29
3
4.9±0.6
15
7.33
4
Eif3cNP_001094132eukaryotic translation initiation factor 3 subunit C
4.6±0.8
7
3.83
4
4.9±0.6
6
4.44
2
Eif3gNP_001013113eukaryotic translation initiation factor 3 subunit G
5.6±1.1
4
2.41
4
5.4±1.2
3
1.97
2
Eif3hNP_942046eukaryotic translation initiation factor 3 subunit H
4.3
1
2.02
4
3.4
1
4.38
4
Eno1NP_001103378alpha-enolase
2.1±0.4
13
4.54
4
0.9±0.1
10
5.7
4
Erlin2NP_001099558erlin-2
6.7±0.4
6
6.74
4
5.1±0.2
4
5.12
4
Fam129bNP_001103355niban-like protein 1
5.6±0.4
3
3.12
3
3.0
3
S
4
FlnbNP_001100758filamin-B
4.4±0.2
42
26.49
3
4.3±0.2
37
25.87
4
Fxyd2NP_663769sodium/potassium-transporting ATPase subunit gamma isoform a
INF
1
-
3
5.8
1
S
4
GapdhNP_058704glyceraldehyde-3-phosphate dehydrogenase
2.7±0.5
9
4.03
4
2.8±0.6
10
4.02
4
Gnai2NP_112297guanine nucleotide-binding protein G(i) subunit alpha-2
4.6±1.2
5
2.17
4
5.4±1.2
6
2.93
4
Gnai3NP_037238guanine nucleotide-binding protein G(k) subunit alpha
4.0±2.2
4
NS
4
4.8±1.1
6
2.76
4
GnasNP_062005GNAS isoform GNASL
6.0±0.6
7
5.52
4
5.3±0.4
6
6.08
4
Gnb2NP_112299guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2
3.6±1.4
4
1.37
4
6.2
4
S
4
Got2NP_037309aspartate aminotransferase, mitochondrial
4.6
2
-
3
2.4±1.4
3
4.85
4
Hsp90aa1NP_786937heat shock protein HSP 90-alpha
2.8±0.4
14
7.4
4
3.0±0.3
12
7.97
4
Hsp90ab1NP_001004082heat shock protein HSP 90-beta
3.2±0.3
20
12.81
4
3.2±0.2
21
18.27
4
Hsp90b1NP_001012197endoplasmin precursor
2.8±0.5
9
4.5
4
3.8±0.5
11
6.4
4
Hspa1aNP_114177heat shock 70 kDa protein 1A/1B
2.8±0.3
24
10.38
4
3.3±0.3
23
14.68
4
Hspa1bNP_997669heat shock 70kD protein 1B
2.8±0.3
24
10.38
4
3.3±0.3
23
14.68
4
Hspa2NP_068635heat shock-related 70 kDa protein 2
3.6±0.4
23
9.08
4
4.1±0.3
16
12.43
4
Hspa5NP_03721578 kDa glucose-regulated protein precursor
2.2±0.5
14
3.53
4
2.5±0.4
12
5.89
4
Hspa8NP_077327heat shock cognate 71 kDa protein
3.0±0.4
21
8.61
4
3.1±0.3
13
10.2
4
Hspd1NP_07156560 kDa heat shock protein, mitochondrial
3.4±0.7
8
3.41
4
2.7±0.4
8
5.26
4
Idh1NP_113698isocitrate dehydrogenase [NADP] cytoplasmic
2.7±0.9
8
2.19
4
0.7±0.3
3
NS
4
ImmtNP_001030100mitochondrial inner membrane protein
5.7±0.4
8
7.9
4
5.0±0.4
6
6
4
Iqgap1NP_001101959ras GTPase-activating-like protein IQGAP1
4.5±0.3
23
18.39
4
3.9±0.3
23
16.54
3
Isyna1NP_001013902inositol-3-phosphate synthase 1
4.3±0.8
10
4.28
4
3.8±0.3
11
9.81
4
Itga2XP_345157integrin alpha-2
6.0±0.9
8
4.59
4
6.3±1.1
11
4.58
4
Itgb1NP_058718integrin beta-1 precursor
5.2±1.6
8
2.47
4
4.9±0.7
9
5.14
4
Kif13bNP_998791kinesin-like protein KIF13B
5.5±0.6
9
6.12
4
4.1±0.9
6
2.95
3
Krt18NP_446428keratin, type I cytoskeletal 18
3.8±0.3
24
17.81
4
3.7±0.3
26
15.88
4
Krt19NP_955792keratin, type I cytoskeletal 19
1.8±0.5
21
3.47
4
2.7±0.4
18
6.14
4
Krt7NP_001041335keratin, type II cytoskeletal 7
2.6±0.7
11
2.75
4
3.3±0.6
13
4.83
4
Krt8NP_955402keratin, type II cytoskeletal 8
2.6±0.4
27
6.47
4
2.7±0.3
32
10.22
4
LdhaNP_058721L-lactate dehydrogenase A chain
2.5±0.4
7
4.31
4
1.9±0.5
6
2.34
4
LnpepNP_001106874leucyl-cystinyl aminopeptidase isoform 1
2.6±0.6
13
3.41
4
4.6±0.4
10
7.49
4
LrbaNP_001102025lipopolysaccharide-responsive and beige-like anchor protein
5.1±0.3
56
28.92
4
4.9±0.3
59
30.8
4
Map7NP_001099740ensconsin isoform 1
4.2
1
-
2
4.5
1
8.78
4
Myh10NP_113708myosin-10
3.6±0.4
19
10.89
4
2.6±0.7
13
3.26
4
Myh9NP_113708myosin-9
2.8±0.4
25
8.29
4
2.3±0.4
19
6.38
4
Myl6NP_001102954myosin light polypeptide 6
2.8±0.1
3
3.9
4
2.7
1
2.52
4
Myo6XP_008764648unconventional myosin-VI
5.5±0.3
10
9.19
4
2.9±0.9
6
1.97
4
MyofNP_001178565myoferlin isoform X5
4.2±0.5
19
9
4
3.5±0.3
25
14.24
4
Ndrg1NP_001011991protein NDRG1
2.2±3.2
3
NS
4
4.7±0.7
3
2.49
4
Pdcd6ipNP_001025081programmed cell death 6-interacting protein
4.0±0.8
6
3.03
3
3.9±0.7
6
3.48
4
Pdia3NP_059015protein disulfide-isomerase A3 precursor
4.4±0.7
14
6.07
4
5.1±0.6
15
7.5
4
Phb2NP_001013053prohibitin-2
0.9±3.2
3
NS
3
4.9
2
3.82
4
PkmNP_445749pyruvate kinase PKM
3.9±0.3
27
17.29
4
3.5±0.1
26
27.21
4
Plxnb2NP_001101576plexin-B2 precursor
2.1
17
S
4
3.5±0.6
17
5.7
3
PplNP_001100446periplakin
3.8±0.5
17
NS
2
3.3±0.4
15
7.81
4
Prdx3NP_071985thioredoxin-dependent peroxide reductase, mitochondrial precursor
6.1±1.2
6
3.08
4
8.4±0.5
5
6.78
4
PurbNP_001017503transcriptional activator protein Pur-beta
3.5
1
2.89
4
INF
1
S
4
PygbNP_037320glycogen phosphorylase, brain form
3.1±0.4
16
8.25
4
2.9±0.4
19
6.92
4
PyglNP_071604glycogen phosphorylase, liver form
3.6±0.1
4
5.52
4
2.2±0.3
3
2.21
3
Rab11aNP_112414ras-related protein Rab-11A
5.6±1.5
4
1.88
4
4.7±1.7
4
1.44
4
Rab11bNP_116006ras-related protein Rab-11B
5.6±1.5
4
1.88
4
4.7±1.7
4
1.44
4
Rab14NP_446041ras-related protein Rab-14
5.0±1.5
5
1.88
4
6.8±0.4
4
5.1
4
Rab17NP_001102833ras-related protein Rab-17
INF
3
-
3
4.8
3
S
4
Rab1aNP_112352ras-related protein Rab-1A
4.3
3
S
4
7.8±0.1
4
8.24
3
Rab2aNP_113906ras-related protein Rab-2A
3.2
2
S
4
5.5
2
-
3
Rab5bNP_001073405ras-related protein Rab-5B
6.0±0.7
4
3.16
4
4.1±1.5
4
1.46
4
Rab5cNP_001099310ras-related protein Rab-5C
4.2±0.6
4
2.85
4
6.8±0.4
4
4.44
4
Rab7aNP_076440ras-related protein Rab-7a
1.7±0.6
4
1.53
4
2.9±0.6
4
2.35
4
Rap1aNP_001005765ras-related protein Rap-1A precursor
4.1±0.7
3
2.36
4
6.1±0.4
3
3.31
4
Rap1bNP_599173ras-related protein Rap-1b precursor
4.1±0.7
3
2.36
4
6.1±0.4
3
3.31
4
Rpl3NP_94204860S ribosomal protein L3
3.8±0.6
4
3.07
4
3.6±0.5
4
3.41
4
Rpn2NP_113886dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 precursor
3.5±0.5
5
3.49
3
4.2±0.5
8
5.86
4
Rps14NP_07316340S ribosomal protein S14
4.3
2
2.92
4
4.6
2
2.86
4
Rps3NP_00100923940S ribosomal protein S3
1.9±1.3
5
NS
4
5.2±0.7
6
4.34
4
Rras2NP_001013452ras-related protein R-Ras2
4.7
1
S
4
6.2
1
-
3
Rtn4NP_114019reticulon-4
4.5±0.3
8
8.73
4
3.0±0.4
7
5
4
S100a11NP_001004095protein S100-A11
2.7
2
-
3
2.0
2
5.72
4
S100a16NP_001102027protein S100-A16
INF
1
S
4
7.6
1
-
3
S100a4NP_036750protein S100-A4
5.2±0.8
4
2.87
3
3.6±0.3
3
3.38
4
Sema4bNP_001163933semaphorin 4B precursor
4.3±0.1
5
6.79
4
2.9±0.9
8
2.25
4
Sept7NP_001107212septin-7 isoform b
6.6±0.0
5
10.92
4
3.0±1.5
6
NS
4
Serbp1NP_659554plasminogen activator inhibitor 1 RNA-binding protein
2.3
2
3.21
4
2.9±0.7
3
1.84
4
Slc14a2NP_001103740urea transporter 2 isoform 3
3.1±1.0
9
2.17
3
4.1±0.9
8
3.29
4
Slc25a24NP_001121016calcium-binding mitochondrial carrier protein SCaMC-1
2.5±0.7
3
1.52
4
4.0±0.8
4
2.36
3
Slc25a3NP_620800phosphate carrier protein, mitochondrial isoform 1
5.1
1
1.66
4
5.5
1
S
4
Slc25a5NP_476443ADP/ATP translocase 2
4.1±0.5
6
5.33
4
4.5±0.7
6
4.31
4
Sptan1NP_741984spectrin alpha chain, non-erythrocytic 1
3.7±0.2
63
40.45
4
3.0±0.2
62
29.12
4
Sptbn1NP_001013148spectrin beta chain, brain 1
4.0±0.5
14
7.67
3
2.7±0.2
9
7.7
4
Sptbn2NP_062040spectrin beta chain, non-erythrocytic 2
4.6±0.2
55
39.17
4
4.4±0.1
49
46.72
4
Tmem30aNP_001004248cell cycle control protein 50A
5.1
3
S
4
5.7
2
2.51
4
Tuba1cNP_001011995tubulin alpha-1C chain
4.3±0.2
20
19.36
4
3.9±0.3
21
15.83
4
Tuba4aNP_001007005tubulin alpha-4A chain
4.2±0.4
18
12.46
4
4.4±0.2
19
18.21
4
Tubb4bXP_006233644tubulin beta-4B chain isoform X1
4.0±0.3
26
14.89
4
4.0±0.3
27
19.03
4
Tubb5NP_775125tubulin beta-5 chain
3.9±0.3
24
16.2
4
4.1±0.2
29
23.03
4
Tubb6NP_001020846tubulin beta-6 chain
4.5±0.6
14
7.31
4
4.5±0.3
13
12.9
4
Uqcrc2NP_001006971cytochrome b-c1 complex subunit 2, mitochondrial precursor
3.3±0.3
6
5.1
4
3.7±0.4
5
4.47
4
Vat1NP_001028855synaptic vesicle membrane protein VAT-1 homolog
4.1±0.8
12
4.31
4
3.5±0.5
11
5.91
4
Vps26bNP_001100279vacuolar protein sorting 26 homolog B
3.9±1.4
5
1.64
4
5.3±1.1
4
2.43
4
Vps35NP_001099188maternal embryonic message 3
5.4±0.5
24
11.86
4
6.0±0.5
23
13.78
4
YwhaqNP_03718514-3-3 protein theta
1.5±0.8
3
NS
4
4.6±0.8
4
2.79
4
YwhazNP_03714314-3-3 protein zeta/delta
2.3±0.2
4
3.49
4
3.9±0.7
4
2.76
4

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