Vasopressin-Regulated Phosphorylation Sites in Collecting Duct
Brief methods: Our prior studies using quantitative phosphoproteomics have identified phosphorylation changes in cultured mouse mpkCCD cells (PMID: 33346914) and native inner medullary collecting duct cells (PMID: 31313956). Here, we have curated a list of vasopressin regulated phosphoproteins based on a consensus of the two studies. This includes 51 phosphorylation sites in 45 proteins.
This website was created by Euijung Park, Joe Chou, Chin-Rang Yang and Mark Knepper. Contact us with questions or comments: knep@helix.nih.gov. Database updated: November 29, 2021. Please cite: Park et al., 2023, PMID:36264882.
⚠ Government Shutdown Notice
Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit https://cc.nih.gov/. Updates regarding government operating status and resumption of normal operations can be found at OPM.gov
Search Using Browser "Find" Command
| Gene Symbol | Annotaion | Rat UniProt No. | Rat Mod. Site | Mouse UniProt No. | Mouse Mod. Site | Direction of Change | Rat Centralized Sequence | Mouse Centralized Sequence | Molecular Function |
| Agfg1 | arf-GAP domain and FG repeat-containing protein 1 | Q4KLH5 | S181 | Q8K2K6 | S181 | decreased | KGTPTQS*PVVGRS | KGTPSQS*PVVGRS | regulation of GTPase activity |
| Akap12 | A-kinase anchor protein 12 | Q5QD51 | S469 | Q9WTQ5 | S467 | decreased | TQLTDLS*PEEKTL | TQLTDLS*PEEKML | cyclic AMP dependent signaling |
| Als2 | alsin | P0C5Y8 | S486 | Q920R0 | S486 | decreased | GLLSQVS*PRLLRK | GLLSQVS*PRLLRK | regulation of GTPase activity |
| Aqp2 | aquaporin-2 | P34080 | S261 | P56402 | S261 | decreased | QSVELHS*PQSLPR | QSVELHS*PQSLPR | transmembrane transport |
| Aqp2 | aquaporin-2 | P34080 | S256 | P56402 | S256 | increased | EVRRRQS*VELHSP | EVRRRQS*VELHSP | transmembrane transport |
| Arfgef1 | brefeldin A-inhibited guanine nucleotide-exchange protein 1 | D4A631 | S1566 | G3X9K3 | S1566 | decreased | DAISQKS*VDIHDS | DAISQKS*VDIHDS | regulation of GTPase activity |
| Arhgef2 | rho guanine nucleotide exchange factor 2 | Q5FVC2 | S885 | Q60875 | S885 | increased | LDPRRRS*LPAGDA | LDPRRRS*LPAGDA | regulation of GTPase activity |
| Bin1 | myc box-dependent-interacting protein 1 | O08839 | S304 | O08539 | S304 | increased | SPPPDGS*PAATPE | SPPPDGS*PAATPE | vesicle-mediated transport |
| Bloc1s5 | biogenesis of lysosome-related organelles complex 1 subunit 5 | B2GV52 | S25 | Q8R015 | S23 | increased | GGKKRDS*LGTAGA | GGKKRDS*LGTPGA | vesicle-mediated transport |
| Borcs6 | BLOC-1-related complex subunit 6 | Q66H43 | S173 | Q9D6W8 | S173 | increased | RLQDSRS*LDGLSG | RLQDSRS*LDGLSG | vesicle-mediated transport |
| Camkk2 | calcium/calmodulin-dependent protein kinase kinase 2 | O88831 | S494 | Q8C078 | S495 | increased | TMIRKRS*FGNPFE | TMIRKRS*FGNPFE | protein phosphorylation |
| Camkk2 | calcium/calmodulin-dependent protein kinase kinase 2 | O88831 | S510 | Q8C078 | S511 | increased | REERSLS*APGNLL | REERSLS*APGNLL | protein phosphorylation |
| Cdk18 | cyclin-dependent kinase 18 | O35832 | S66 | Q04899 | S66 | increased | QNQRRFS*MEDLNK | QNQRRFS*MEDLNK | protein phosphorylation |
| Clip1 | CAP-Gly domain-containing linker protein 1 | Q9JK25 | S347 | Q922J3 | S347 | increased | RYARKIS*GTTALQ | RYARKIS*GTTALQ | microtubule cytoskeleton |
| Ctnnb1 | catenin beta-1 | Q9WU82 | S552 | Q02248 | S552 | increased | DTQRRTS*MGGTQQ | DTQRRTS*MGGTQQ | regulation of transcription |
| Ctnnb1 | catenin beta-1 | Q9WU82 | T551 | Q02248 | T551 | increased | QDTQRRT*SMGGTQ | QDTQRRT*SMGGTQ | regulation of transcription |
| Erbb3 | receptor tyrosine-protein kinase erbB-3 | Q62799 | S980 | Q61526 | S980 | increased | LVIKRAS*GPGTPP | LVIKRAS*GPGIPP | protein phosphorylation |
| Exoc7 | exocyst complex component 7 | O54922 | S250 | O35250 | S250 | decreased | SSGVPYS*PAIPNK | SSGVPYS*PAIPNK | vesicle-mediated transport |
| Hdac4 | histone deacetylase 4 | Q99P99 | S245 | Q6NZM9 | S245 | decreased | PLRKTAS*EPNLKL | PLRKTAS*EPNLKL | regulation of transcription |
| Igsf5 | immunoglobulin superfamily member 5 | Q5VJ70 | S334 | Q7TSN7 | S335 | increased | LPEQRSS*LPQQEL | PEKRSSS*LPYQEL | cell polarity |
| Itpr1 | inositol 1,4,5-trisphosphate receptor type 1 | P29994 | S1589 | P11881 | S1588 | increased | NAARRDS*VLAASR | NAARRDS*VLAASR | calcium signaling |
| Itpr1 | inositol 1,4,5-trisphosphate receptor type 1 | P29994 | S1756 | P11881 | S1755 | increased | PSGRRES*LTSFGN | PSGRRES*LTSFGN | calcium signaling |
| Itpr2 | inositol 1,4,5-trisphosphate receptor type 2 | P29995 | S1709 | Q9Z329 | S1709 | increased | YFKGDHS*VGVNGP | YFKGDHS*ISVNGP | calcium signaling |
| Itpr3 | inositol 1,4,5-trisphosphate receptor type 3 | Q63269 | S1832 | P70227 | S1832 | increased | TKGRVSS*FSMPSS | TKGRVSS*FSMPSS | calcium signaling |
| Itsn1 | intersectin-1 | Q9WVE9 | S315 | Q9Z0R4 | S315 | increased | RRVRSGS*GMSVIS | RRVRSGS*GMSVIS | vesicle-mediated transport |
| Lrrfip1 | leucine-rich repeat flightless-interacting protein 1 | Q66HF9 | S88 | Q3UZ39 | S88 | increased | TSSRRGS*GDTSIS | TSSRRGS*GDTSIS | regulation of transcription |
| Luzp1 | leucine zipper protein 1 | Q9ESV1 | S261 | Q8R4U7 | S261 | increased | ESRRKGS*LDYLKQ | ESKRKGS*LDYLKQ | cell polarity |
| Mink1 | misshapen-like kinase 1 | F1LP90 | S782 | Q9JM52 | S746 | decreased | STKLDSS*PVLSPG | STKLDSS*PVLSPG | protein phosphorylation |
| Myo9b | unconventional myosin-IXb | Q63358 | S1649 | Q9QY06 | S1647 | increased | YTGRRKS*ELGAEP | YTGRRKS*ELGAEP | actin cytoskeleton |
| Nsfl1c | NSFL1 cofactor p47 | O35987 | S176 | Q9CZ44 | S176 | increased | GERRRHS*GQDVHV | GERRRHS*GQDVHV | vesicle-mediated transport |
| Pde7a | high affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A | O08593 | S28 | P70453 | S58 | increased | ETERRGS*HPYIDF | ETERRGS*HPYIDF | cyclic AMP dependent signaling |
| Pdlim5 | PDZ and LIM domain protein 5 isoform ENH3a | Q62920 | S111 | Q8CI51 | S111 | decreased | KPVPITS*PAVSKV | KPVPITS*PAVSKV | actin cytoskeleton |
| Pi4kb | phosphatidylinositol 4-kinase beta | O08561 | S511 | Q8BKC8 | S511 | increased | DIRRRLS*EQLAHT | DIRRRLS*EQLAHT | calcium signaling |
| Prkar2a | cAMP-dependent protein kinase type II-alpha regulatory subunit | P12368 | S97 | P12367 | S96 | decreased | KFTRRVS*VCAETF | KFTRRVS*VCAETF | cyclic AMP dependent signaling |
| Prrc2a | protein PRRC2A | Q6MG48 | S455 | Q7TSC1 | S454 | increased | RQRRKQS*SSEISL | RQRRKQS*SSEISL | RNA processing |
| Prrc2a | protein PRRC2A | Q6MG48 | T783 | Q7TSC1 | T782 | decreased | LLRERGT*PPVDPK | LLRERGT*PPVDPK | RNA processing |
| Ralgapa2 | ral GTPase-activating protein subunit alpha-2 | P86411 | S820 | A3KGS3 | S820 | decreased | LVRRSSS*PAELDL | LVRRSSS*PAELEL | regulation of GTPase activity |
| Rmdn3 | regulator of microtubule dynamics protein 3 | Q66H15 | S46 | Q3UJU9 | S46 | increased | RHGRSQS*LPNSLD | RHGRSHS*LPNSLD | microtubule cytoskeleton |
| Sec22b | vesicle-trafficking protein SEC22b | Q4KM74 | S137 | O08547 | S137 | increased | ARRNLGS*INTELQ | ARRNLGS*INTELQ | vesicle-mediated transport |
| Sept9 | septin-9 | Q9QZR6 | T125 | Q80UG5 | T143 | decreased | EVLGHKT*PEPVPR | EVLGHKT*PEPVPR | cell polarity |
| Slc9a3r1 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | Q9JJ19 | S277 | P70441 | S275 | decreased | VEPASES*PRPALA | VEPASES*PRPALA | cell polarity |
| Specc1l | cytospin-A | Q2KN99 | S385 | Q2KN98 | S385 | increased | ERSRKGS*SGNASE | ERSRKGS*SGNASE | actin cytoskeleton |
| Specc1l | cytospin-A | Q2KN99 | S833 | Q2KN98 | S833 | increased | GLSRRSS*TSSEPT | GLSRRSS*TSSEPT | actin cytoskeleton |
| Src | neuronal proto-oncogene tyrosine-protein kinase Src | Q9WUD9 | S17 | P05480 | S17 | increased | ASQRRRS*LEPAEN | ASQRRRS*LEPSEN | protein phosphorylation |
| Stim1 | stromal interaction molecule 1 isoform 2 | P84903 | S575 | P70302 | S575 | decreased | VEKLPDS*PALAKK | VEKLPDS*PALAKK | calcium signaling |
| Stxbp5 | syntaxin-binding protein 5 | Q9WU70 | S760 | Q8K400 | S760 | increased | KMSRKLS*LPTDLK | KMSRKLS*LPTDLK | vesicle-mediated transport |
| Tanc1 | protein TANC1 | Q6F6B3 | S1658 | Q0VGY8 | S1665 | decreased | SSGSSGS*PSSSVK | SSGSSGS*PSSSIK | cell polarity |
| Tsc22d4 | TSC22 domain family protein 4 | Q3B8N7 | T223 | Q9EQN3 | T223 | decreased | GGLAAGT*PPLSRR | GGSAAAT*PPLSRR | regulation of transcription |
| Veph1 | ventricular zone-expressed PH domain-containing protein 1 | Q5PQS3 | S430 | A1A535 | S430 | increased | GSVRRYS*LDHVSK | GSGRRYS*LDHISK | regulation of transcription |
| Wdr7 | WD repeat-containing protein 7 | Q9ERH3 | S935 | Q920I9 | S935 | decreased | LSKARDS*PPASSN | LSKARDS*PPPSSN | vesicle-mediated transport |
| Zfp36l1 | mRNA decay activator protein ZFP36L1 | P17431 | S54 | P23950 | S54 | increased | GFPRRHS*VTLPSS | GFPRRHS*VTLPSS | RNA processing |