This database provides differential expression analysis (EdgeR) of RNA-seq data from ATF1/CREB1/CREM triple knockout mpkCCD cells(tKO), with rescue of individual transcription factors versus control tKO cells. FDR, false discovery rate.

Brief Method: Triple KO mpkCCD cells underwent lentiviral transduction of each of the individual TFs (ATF1, CREB1, and CREM). Total RNA was isolated from three biological replicates of each corresponding group using a Direct-zol RNA MiniPrep Plus Kit (Zymo Research) following the manufacturer’s protocol. First strand cDNA was prepared using 40 ng of total RNA using SMART-Seq® mRNA Kit, followed by cDNA amplification. After purification of amplified cDNA using the AMPure XP beads (A63880, Beckman Coulter), the concentration of the synthesized cDNA was examined by Qubit dsDNA HS DNA assay kit (Q32851, Invitrogen). One nanogram of the synthesized cDNAs were fragmented and tagged with index primers (FC-131-1024, Nextera XT DNA library Preparation Kit, Illumina) following the manufacturer’s protocol. RNA-seq was performed by 50-bp paired-end NovaSeq (Illumina). Ten samples were loaded in each lane. Raw sequencing reads were aligned by STAR 2.7.10 b to the mouse reference genome (Ensembl genome 106). Transcript per million and expected counts were generated by RSEM 1.3.1. Expected counts were used for differential gene expression analysis using edgeR 3.38.4

Reference: Murillo-de-Ozores AR, Chen L, Ou SM, Park E, Khan S, Raghuram V, Yang CR, Chou CL, Knepper MA. CREB-Family Transcription Factors and Vasopressin-Mediated Regulation of Aqp2 Gene Transcription. J Am Soc Nephrol. 2026; 37: 67-84. PMID: 40857118.

This website was created by Adrian Murillo de Ozores and Mark Knepper. For questions or comments: knep@helix.nih.gov.

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