This database lists changes in abundance for expressed mRNAs in mouse collecting duct cells (mpkCCD) resulting from deletion of the protein kinase “Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A” (Dyrk1a). Dyrk1a is an inhibitor of entry into the cell cycle via activation of the DREAM complex. Quantification used RNA-seq in four independent clones with CRISPR-Cas9-induced knockout of Dyrk1a (“Dyrk1a-KO”) compared with four clones without deletion of Dyrk1a (“WT”). Cells were exposed to the vasopressin analog dDAVP (0.1 nM) for 48 hours prior to harvest. FDR-corrected P values (P_adj less than 0.01 are indicated in bold. Among the transcripts with P_adj less than 0.01, log₂₍dDAVP/vehicle) values greater than 0.5 are indicated in green, while those less than -0.5 are indicated in red. Abbreviations: KO,knockout; WT, without transformation; TPM, transcripts per million.

Database was created by Euijung Park, Lihe Chen, and Mark Knepper in the Epithelial Systems Biology Laboratory (Mark Knepper, Chief) at the National Heart, Lung and Blood Institute as part of its Kidney Systems Biology Project. Reference: Pending. Click to download processed data as spreadsheet.

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