National Heart, Lung, and Blood Institute

Epithelial Systems Biology Laboratory (ESBL)


IMCD Phosphoproteomics Data on Hypercalcemia Induced Nephrogenic Diabetes Insipidus

This database represents renal inner medullary collecting duct (IMCD) proteins and phosphoproteins from PTH treated rats and controls at the early stage of hypercalcemia-induced NDI. Male Sprague-Dawley rats were treated with PTH (15 μg/kg/day) or vehicle via osmotic minipump for 1 day. All data are from IMCD cells freshly isolated from rat renal inner medullas.

Brief methods: Samples were analyzed using LTQ Orbitrap XL mass spectrometer equipped with nanoflow ultra-high pressure liquid chromatography system. The mass spectra were analyzed using two search algorithms, SEQUEST (Bioworks version 3.31 SP1) and InsPecT (version 20070905).

The work was performed at the Faculty of Medicine, Thammasat University, and the Faculty of Medicine, Chulalongkorn University, Thailand. This database was created by Sookkasem Khositseth and Trairak Pisitkun. Contact us for any questions: pisitkut@nhlbi.nih.gov Database is hosted by the Epithelial Systems Biology Laboratory (Mark Knepper, Chief) at the National Heart, Lung, and Blood Institute as part of its Kidney Systems Biology Project.

Reference: Khositseth S, Charngkaew K, Boonkrai C, Somparn P, Uawithya P, Chomanee N, Payne DM, Fenton RA, Pisitkun T. Hypercalcemia induces targeted autophagic degradation of aquaporin-2 at the onset of nephrogenic diabetes insipidus. Kidney Int. 2017 May;91(5):1070-1087. PMID:28139295

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Phosphoprotein Quantification

Masterlist  Protein Name Phosphorylation
Site(s)		 Average
Log2(PTH/Control)		 SE P-value
NP_001101634 Cgnl1 S251 -1.56 0.27 0.00
NP_112383 Ssrp1 S444 -0.67 0.21 0.03
NP_001101634 Cgnl1 S283 -1.64 0.54 0.04
NP_114013 Fbn1 S2703 -1.12 0.42 0.04
NP_037041 Aqp2 S261 -0.84 0.28 0.04
NP_997707 Ppp1r11 S78 -0.91 0.32 0.04
NP_037041 Aqp2 S256 -1.19 0.47 0.04
NP_001157780 Plec S21 -0.68 0.27 0.05
NP_001099350 Zbtb20 S359 -1.04 0.42 0.05
NP_062137 Prkar2a S97 -0.68 0.28 0.05
NP_001101634 Cgnl1 S198,S202 -1.49 0.54 0.05
NP_851597 Arfgef2 S218,S227 -1.00 0.37 0.05
NP_073636 Nucks1 S19 -0.56 0.23 0.05
NP_037041 Aqp2 S256,S261 -0.94 0.40 0.06
NP_001013225 Rbm39 S136 -0.74 0.33 0.06
NP_446368 Trim28 S474 -0.75 0.36 0.08
NP_001012147 Pxn S317 -0.90 0.44 0.09
NP_036784 Slc9a1 S790 -0.77 0.38 0.09
NP_001013148 Sptbn1 S2154,S2159 -1.00 0.49 0.09
NP_001103067 Cys1 T55 -1.33 0.61 0.10
NP_446159 Hdgf S202 -1.25 0.63 0.10
XP_001056343 Srrm2 S1218 -1.25 0.65 0.10
NP_001019962 Epn3 S419 -0.61 0.32 0.10
NP_001101855 Epb49 S226 -0.46 0.24 0.10
NP_001012147 Pxn T313 -0.80 0.42 0.10
NP_001001515 Lmo7 S652 -0.42 0.20 0.11
NP_068640 Cttn T364,S381 -0.78 0.42 0.11
NP_598249 G3bp1 S229 -1.03 0.51 0.11
NP_068522 Nop58 S507 -0.88 0.44 0.11
NP_037019 Tmpo T74 -1.00 0.50 0.12
NP_001103022 Sfrs1 S199 -2.79 1.52 0.12
NP_001034100 Cnksr1 T284,S288 -0.73 0.40 0.12
NP_446225 Tjp2 S1132 -0.91 0.51 0.12
NP_001094132 Eif3c S39 -0.97 0.50 0.13
NP_598249 G3bp1 S231 -0.85 0.48 0.13
NP_058686 Add1 T610 -0.93 0.53 0.13
NP_476482 Hnrnpk T118 -1.46 0.83 0.13
NP_114182 Prkab1 S108 -2.37 1.24 0.13
NP_001001515 Lmo7 S1476 -0.56 0.32 0.13
NP_001013452 Rras2 S186 -0.98 0.52 0.13
NP_851597 Arfgef2 S227 -0.70 0.37 0.13
NP_001028240 Edc4 T732 -1.08 0.63 0.14
NP_001186244 Gtpbp1 S8 -0.70 0.41 0.14
NP_742005 Canx S553,T561 -1.15 0.62 0.14
NP_001102918 LOC683897 S106 -0.37 0.22 0.14
NP_112338 Pcm1 S65 -0.82 0.45 0.14
NP_001028240 Edc4 S734 -0.84 0.50 0.14
NP_001013249 Pea15a S116 -0.97 0.58 0.15
NP_001129138 Lrrc47 S292 -0.65 0.39 0.15
NP_001001515 Lmo7 S650 -0.73 0.44 0.15
NP_037349 Mllt4 S1189 -0.86 0.52 0.15
NP_001009720 Sfrs2 T25 -0.72 0.40 0.15
NP_476489 Sept2 S218 -0.46 0.29 0.16
NP_037326 Myh9 S1944 -0.61 0.38 0.16
NP_476482 Hnrnpk S116 -1.40 0.88 0.16
NP_665715 Dync1li1 S516 -0.94 0.59 0.16
NP_067605 Slc9a3r1 S287 -0.75 0.47 0.16
NP_001004082 Hsp90ab1 S255 -0.57 0.36 0.16
NP_001019449 Map4 S520 -0.45 0.29 0.17
NP_071783 Fxyd4 S71 -1.13 0.69 0.18
NP_001008375 Pgrmc2 S202 -0.75 0.46 0.18
NP_001094189 Ap3d1 S760 -0.74 0.46 0.18
NP_001008375 Pgrmc2 T205 -0.47 0.31 0.18
NP_001013148 Sptbn1 S2097 -1.53 1.02 0.18
NP_001026810 Eif3b S75,S84 -0.71 0.47 0.18
NP_620240 Golgb1 S498 -0.59 0.37 0.19
NP_114019 Rtn4 S107 -0.75 0.48 0.19
NP_001101543 Tjp3 S348 -0.57 0.36 0.19
NP_446159 Hdgf S165 -0.60 0.41 0.19
NP_001019962 Epn3 S175 -1.04 0.67 0.19
NP_001012488 Pqcp S534 -0.67 0.46 0.19
NP_062022 Matr3 S598 -1.44 0.99 0.20
NP_001020804 Hnrnpc S226 -1.13 0.78 0.20
NP_001026810 Eif3b S75,S79 -0.66 0.46 0.20
NP_001103611 Eif5b S215 -0.62 0.41 0.20
NP_037030 Ahsg S138 -0.58 0.41 0.20
NP_958819 Zc3h18 S91 -0.62 0.41 0.20
NP_067695 Nucb2 S89 0.81 0.56 0.22
NP_001100083 Ppp4r2 S280 -1.25 0.91 0.22
NP_001101412 Lad1 S181 -2.50 1.73 0.22
NP_001101456 Srrm1 S220 -0.76 0.54 0.23
XP_001056343 Srrm2 S295,S321 -0.79 0.56 0.23
NP_001019932 Lrrfip2 S133 -0.41 0.31 0.23
NP_620240 Golgb1 S496 -0.55 0.39 0.24
NP_001163939 Acin1 S216 -0.52 0.40 0.24
NP_001008375 Pgrmc2 Y204 -0.67 0.49 0.24
NP_001020897 Srpk1 S51 -0.69 0.51 0.24
NP_001029301 Sec62 T365 -1.43 1.07 0.25
NP_001011958 Raly S277 -0.56 0.45 0.25
NP_058944 Hnrnpa1 S6 -1.85 1.47 0.25
NP_114182 Prkab1 S182 -0.73 0.55 0.26
NP_001101879 Fgd3 S7 -0.61 0.49 0.26
NP_001019971 Tmx1 S245 -0.73 0.55 0.26
NP_068640 Cttn T364,S370 -0.91 0.69 0.26
NP_001013920 RGD1311703 S17 -0.55 0.42 0.26
NP_068640 Cttn T364,S380 -0.63 0.51 0.26
NP_001099736 Tjp1 S113,Y120 -2.63 2.03 0.27
NP_446225 Tjp2 S239 -1.05 0.86 0.27
NP_001102977 LOC688717 S105 -0.47 0.36 0.27
NP_786937 Hsp90aa1 S263 -0.68 0.56 0.27
NP_001163943 RGD1309922 S612 -0.54 0.45 0.27
NP_001014068 Fam82a2 S46 -0.82 0.64 0.27
NP_001094189 Ap3d1 S784 -0.71 0.56 0.28
NP_598249 G3bp1 S149 -0.60 0.50 0.28
NP_001030332 Sfrs11 S427 -0.60 0.48 0.28
NP_037313 Prkar1a S83 -0.37 0.30 0.28
NP_001012147 Pxn S316 -0.53 0.43 0.28
NP_114187 Nsfl1c S114 -0.51 0.43 0.28
NP_445915 Nucb1 S85 -1.33 1.13 0.28
NP_742019 Prosapip1 S346 -1.12 0.90 0.28
NP_037042 Arrb1 S412 -0.72 0.59 0.29
NP_001008325 Eif4b S498 -1.84 1.58 0.29
NP_001101456 Srrm1 S401,T404 -0.62 0.51 0.29
NP_001094328 Top2b S1387,S1400 -1.31 1.09 0.30
NP_077075 Dnajc5 S10 -0.98 0.86 0.30
NP_476472 Prpsap2 S227 -0.76 0.64 0.30
NP_446095 Cds2 S32 -0.52 0.44 0.30
NP_001101456 Srrm1 S448 -1.08 0.95 0.30
NP_001100267 Cbx5 S93 -0.56 0.49 0.30
NP_037202 Utrn S933 -0.48 0.42 0.30
NP_037042 Arrb1 T410 -0.72 0.61 0.31
NP_001102025 Lrba S1715 -0.95 0.85 0.31
NP_476480 Hnrnpu S245 -0.83 0.75 0.31
NP_001124037 Fam83h S522 -0.60 0.52 0.32
NP_062090 Map1b S1393 -0.86 0.78 0.32
NP_001101456 Srrm1 S877 -0.50 0.43 0.32
NP_612512 Map3k2 S164 0.16 0.15 0.33
NP_001019962 Epn3 S417,S419 -1.42 1.29 0.33
NP_001009693 Thrap3 S248 -2.27 2.06 0.33
NP_113804 Zranb2 S153 -0.36 0.34 0.33
NP_001094328 Top2b S1387 -0.61 0.56 0.33
NP_001013104 Capg S338 -0.50 0.48 0.34
NP_001009665 Ebag9 S36 -0.38 0.36 0.34
NP_113804 Zranb2 S120 -0.35 0.33 0.34
NP_001001515 Lmo7 T1477 -0.54 0.50 0.34
NP_001124037 Fam83h S860,S871,S882,S893,S904 -0.39 0.38 0.34
NP_001099972 Usp8 S681 -0.57 0.55 0.34
NP_062252 Uso1 S940 -0.54 0.53 0.35
NP_001013148 Sptbn1 S2132 -0.52 0.50 0.35
NP_001041317 Bclaf1 S512 -0.89 0.88 0.35
NP_001009693 Thrap3 S243 -3.56 3.39 0.35
NP_446225 Tjp2 S463 -0.60 0.57 0.35
NP_742005 Canx S582 -0.69 0.67 0.36
NP_001166999 Trappc10 S708 -0.77 0.78 0.38
NP_001029174 Yap1 S46 -0.84 0.88 0.38
NP_001012098 Recql S602 -0.65 0.65 0.38
NP_997684 Tomm70a S94 -0.50 0.51 0.39
NP_001094198 Ccdc43 T137 -0.28 0.31 0.40
NP_001124008 Fzd6 S659 -0.65 0.70 0.41
NP_001128331 Sec23ip S749 -0.70 0.76 0.41
NP_958819 Zc3h18 S63 -0.26 0.29 0.41
NP_001100046 Armc10 S43 -0.37 0.41 0.42
NP_741984 Sptan1 S1217 -0.43 0.48 0.42
NP_001099736 Tjp1 S605 -0.52 0.58 0.42
NP_113708 Myh10 S1956 -0.38 0.44 0.42
NP_001162145 Sntb2 S92 -3.15 3.56 0.43
NP_001102397 Osbp S226 -0.80 0.95 0.43
NP_114019 Rtn4 S169 -0.30 0.35 0.43
NP_114019 Rtn4 S171 -0.30 0.35 0.43
NP_001103353 Abcf1 S109 -0.46 0.53 0.43
NP_445758 Pak2 S141 -0.36 0.44 0.44
NP_062257 Scaf1 S156 -0.29 0.35 0.44
NP_001014253 Dcaf8 S99 -0.40 0.49 0.45
NP_114019 Rtn4 S764 -1.25 1.50 0.45
NP_001017503 Purb S307 1.47 1.84 0.47
NP_001009601 Klc4 S590 -0.38 0.50 0.48
NP_001121063 LOC687105 S230 -0.36 0.46 0.48
NP_036914 Bckdha S338 -0.40 0.53 0.49
NP_001103353 Abcf1 S140 -0.88 1.17 0.49
NP_077075 Dnajc5 S8 -0.92 1.23 0.49
NP_445915 Nucb1 S368 -0.41 0.54 0.49
NP_001099736 Tjp1 Y120 -0.38 0.53 0.51
NP_446307 Akap8 S659 -0.95 1.34 0.52
NP_001029156 Ank3 S875 0.28 0.42 0.53
NP_113751 Ybx1 S163 -0.38 0.56 0.53
NP_058729 Pgm1 S117 -0.42 0.62 0.53
NP_001041317 Bclaf1 S397 -0.32 0.49 0.54
NP_001034114 Tln1 S2040 -0.19 0.29 0.54
NP_620178 Ppp1r2 S87 -0.25 0.40 0.55
NP_036663 Comt S260 -0.36 0.55 0.55
NP_001012170 Nap1l4 S125 -0.32 0.49 0.55
NP_001099972 Usp8 Y680 -0.51 0.80 0.56
NP_036784 Slc9a1 S697 1.29 2.01 0.56
XP_001056343 Srrm2 S1280 -0.45 0.74 0.56
NP_074060 Nolc1 S567 -0.25 0.40 0.57
NP_803175 Gstm2 S117 -0.30 0.50 0.58
NP_446072 Cdc42bpb S1692 -0.21 0.35 0.59
NP_001009688 Thumpd1 S88 -0.24 0.43 0.59
NP_001013122 Eef1d S531 -0.33 0.60 0.60
NP_001030001 Ubxn1 S188 -0.27 0.49 0.61
NP_071971 Dap S51 -0.35 0.63 0.61
NP_001004082 Hsp90ab1 S226 -0.20 0.38 0.62
NP_113907 Clns1a S100 -0.31 0.59 0.62
NP_001029174 Yap1 T48 -0.43 0.83 0.63
NP_001102025 Lrba S1712 -0.19 0.39 0.64
NP_001008521 Twf1 S143 -0.44 0.90 0.65
NP_001101879 Fgd3 T9 -0.23 0.47 0.65
NP_596889 Mtdh S425 0.13 0.29 0.67
NP_001102269 Eef1b2 S106 -0.29 0.68 0.68
NP_112343 Pdpk1 S244 -0.15 0.36 0.69
NP_001013148 Sptbn1 S2155,S2159 -0.24 0.69 0.74
NP_001005879 Gfpt1 S243 -0.13 0.40 0.77
NP_001011923 Prpf4b Y849 -0.11 0.34 0.77
NP_620793 Phrf1 S993 -0.26 0.86 0.78
NP_001014051 Fam122a S36 0.10 0.34 0.78
NP_062161 App S441 -0.10 0.35 0.80
NP_067605 Slc9a3r1 S285 0.40 1.65 0.82
NP_001101412 Lad1 S62 -0.09 0.43 0.84
NP_001014051 Fam122a S34 0.07 0.36 0.85
NP_073636 Nucks1 S14 0.15 0.77 0.86
NP_001013113 Eif3g S42 -0.26 1.43 0.86
NP_073194 Sgta T81 -0.09 0.53 0.87
NP_446222 Sorbs2 S1113 -0.10 0.68 0.89
NP_001020580 Hdac1 S393 0.06 0.43 0.90
NP_001094359 Shroom3 S438,S442 0.14 1.36 0.92
NP_742005 Canx T561 0.04 0.46 0.93
NP_998791 Kif13b S1629 -0.07 0.73 0.93
NP_001007146 Ctnna1 S643 -0.05 0.55 0.93
NP_001014291 Lrrfip1 S348 -0.03 0.46 0.95
NP_001102754 LOC500797 S255 0.03 0.50 0.96
NP_037124 Npm1 S125 -0.02 0.34 0.96
NP_062021 Strn S245 -0.01 0.26 0.97
NP_445899 Hdac2 S394 0.02 0.57 0.98