National Heart, Lung, and Blood Institute

Epithelial Systems Biology Laboratory (ESBL)


UT-A1 Interacting Proteins in Rat Inner Medullary Collecting Duct

This database reports proteins identified by mass spectrometry (LC-MS/MS) that bound to urea channel protein UT-A1 in rat inner medullary collecting duct. IMCD suspenions were treated with vehicle or vasopressin analog (dDAVP, 1 nM) for 15 minutes, followed by in-cell crosslinking using BSOCOES. After incubations, IMCD were harvested by brief centrifugation. The samples were homogenized and solubilized with RIPA. The detergent solubilized fractions were subjected to immunoprecipitation (IP) using UT-A1 antibody-coated Dynabeads or preimmune IgG-coated beads as control. The IP products were eluted in Laemmli buffer and loaded for SDS-Page. Gel pieces corresponding to UT-A1 high molecular weigh complex band above 250 kDa were cut and prepared for mass spectrometry (LC-MS/MS) analysis according to standard in-gel digestion procedures. Proteins that found in all three experiments and were statistically significant in either control or dDAVP-treated group were included in the interactome list.

Definitions: R, ratio (peptide intensity in UT-A1 antibody specific immunoprecipitation sample)/(peptide intensity in preimmune-IgG immunoprecipitation sample) for each peptide found. INF, infinite value. p-value, P value of student t-test vs log2R=0. -log (P) greater than 1.30 was considered statistically significant. S, significant, P value was not available. NS, not significant. -, not available.

Reference: Chou CL, Hwang G, Hageman DJ, Han L, Agrawal P, Pisitkun T, Knepper MA. Identification of UT-A1- and AQP2-interacting proteins in rat inner medullary collecting duct. Am J Physiol Cell Physiol. 2018;314: C99-C117. PMID: 29046292. Contact: This website was created by Chung-Lin Chou and Mark Knepper. Please address questions or comments to: knep@helix.nih.gov

Database created: December 20, 2016 (download data)

Sort the database by category:

Vehicle
dDAVP
Gene Symbol
Ref-Seq Number
Protein Name
Log2R, Mean±SE
# Peptide
-Log(p-value)
# Exp
Log2R, Mean±SE
# Peptide
-Log(p-value)
# Exp
Abcb7NP_997683ATP-binding cassette sub-family B member 7, mitochondrial
5.9±0.8
9
4.20
3
6.8±0.5
11
7.01
3
Actn4NP_113863alpha-actinin-4
3.7±0.5
7
4.82
3
2.4±0.3
4
3.98
3
AhnakNP_001178880neuroblast differentiation-associated protein AHNAK
5.8±0.4
12
7.92
3
4.7±0.5
12
6.07
3
Akr1b1NP_036630aldose reductase
3.9±0.2
5
4.27
3
3.2±0.2
5
4.11
3
Anxa1NP_037036annexin A1
3.7±0.2
9
7.18
3
3.0±0.3
7
4.28
3
Anxa2NP_063970annexin A2
3.5±0.2
9
7.00
3
3.0±0.1
9
8.38
3
Ap1g1NP_604455AP-1 complex subunit gamma-1
5.0±0.3
3
-
2
4.9±0.5
4
2.69
3
Ap3b1NP_001101116AP-3 complex subunit beta-1
6.2±0.6
3
-
2
4.0±1.3
4
2.12
3
Atg9aNP_001014240autophagy-related protein 9A
5.3±0.5
4
2.67
3
6.3±0.5
6
4.30
3
Atp1a1NP_036636sodium/potassium-transporting ATPase subunit alpha-1 precursor
3.7±0.4
12
6.16
3
1.9±0.1
12
7.46
3
Atp1b1NP_037245sodium/potassium-transporting ATPase subunit beta-1
4.0±1.6
3
-
2
2.3±0.4
3
1.62
3
Atp2a2NP_001103609sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform b
6.4±0.7
6
-
2
4.1±0.4
7
4.26
3
Atp2b1NP_445763plasma membrane calcium-transporting ATPase 1
4.5±1.0
5
1.91
3
4.9±0.7
4
2.26
3
Atp5a1NP_075581ATP synthase subunit alpha, mitochondrial precursor
3.5±0.4
5
-
2
3.4±0.7
8
2.84
3
Atp6v1hNP_001013951V-type proton ATPase subunit H
5.5±0.4
4
3.02
3
5.5±0.9
4
2.00
3
Camk2dNP_036651calcium/calmodulin-dependent protein kinase type II subunit delta
6.0±0.8
4
2.31
3
4.4±0.3
2
2.27
3
CanxNP_742005calnexin precursor
5.5±0.6
8
-
2
5.2±0.9
7
2.98
3
Capn1NP_062025calpain-1 catalytic subunit
4.4±0.6
8
3.93
3
4.2±0.4
8
4.66
3
Cct4NP_877966T-complex protein 1 subunit delta
4.8±1.4
3
-
2
4.1±1.4
4
1.62
3
Cdc42bpbNP_446072serine/threonine-protein kinase MRCK beta
6.0±0.4
4
-
2
5.1±0.9
6
2.54
3
Clic1NP_001002807chloride intracellular channel protein 1
4.4±0.5
3
1.85
3
5.1±1.0
3
-
2
CltcNP_062172clathrin heavy chain 1
5.1±0.3
42
21.38
3
4.7±0.2
39
20.53
3
Cnot1NP_001128312CCR4-NOT transcription complex subunit 1
3.1±1.0
4
NS
3
3.6±0.9
5
1.82
3
CopaNP_001128012coatomer subunit alpha
4.8±0.4
15
7.93
3
4.0±0.3
14
8.28
3
Copb1NP_542959coatomer subunit beta
7.1±0.3
4
-
2
5.3±0.5
6
3.72
3
Copg1NP_001026992coatomer subunit gamma-1
5.4
4
-
3
5.2±0.6
5
4.05
3
Copg2NP_001100399coatomer subunit gamma-2
6.6
3
-
3
4.8±0.8
3
2.4
3
CryabNP_037067alpha-crystallin B chain
6.4±0.5
6
-
2
5.0±0.6
5
2.97
3
Ctps2NP_001030170CTP synthase 2
7.2±0.3
1
2.88
3
3.8±1.1
3
-
2
CttnNP_068640cortactin isoform B
6.3
1
-
1
4.3±0.8
2
1.93
3
Cyfip1NP_001100987cytoplasmic FMR1 interacting protein 1
5.2±0.7
2
2.71
3
4.5±1.1
3
2.02
3
Ddx3xNP_001101716ATP-dependent RNA helicase DDX3X
4.8±0.6
3
1.84
3
6.3±1.5
4
1.63
3
Ddx5NP_001007614probable ATP-dependent RNA helicase DDX5
4.2±0.1
1
3.40
3
3.0±0.6
1
2.06
3
Dock8NP_001032882dedicator of cytokinesis 8
6.8±0.3
24
16.92
3
6.4±0.2
29
21.23
3
Dpysl2NP_001099187dihydropyrimidinase-related protein 2
5.7±0.4
10
6.88
3
4.9±0.2
12
9.50
3
DstnNP_001028838destrin
3.3
1
-
1
2.3±0.5
1
1.42
3
Dync1h1NP_062099cytoplasmic dynein 1 heavy chain 1
5.7±0.3
31
18.37
3
4.7±0.3
25
13.33
3
Eef1a1NP_284925elongation factor 1-alpha 1
5.0±0.2
8
7.19
3
4.5±0.5
9
4.78
3
Eef1gNP_001004223elongation factor 1-gamma
5.4±1.1
2
1.76
3
4.5±0.4
2
2.84
3
Eef2NP_058941elongation factor 2
5.2±0.5
12
6.70
3
5.3±0.8
12
4.35
3
Eml4NP_001101478echinoderm microtubule-associated protein-like 4
8.0
1
-
1
4.3±0.4
1
2.04
3
Eno1NP_001103378alpha-enolase
4.0±0.3
3
-
2
2.0±0.3
5
2.45
3
Epb41l1NP_067713band 4.1-like protein 1 isoform L
5.3
1
-
1
3.4±0.2
1
2.47
3
EprsNP_001019409bifunctional aminoacyl-tRNA synthetase
4.8±0.5
10
5.04
3
4.9±0.2
9
7.64
3
Esyt1NP_058945extended synaptotagmin-1
4.7±0.5
13
6.30
3
3.2±0.8
7
2.13
3
Farp1NP_001100757FERM, RhoGEF and pleckstrin domain-containing protein 1
-
-
-
-
4.2±0.8
1
1.42
3
FlnaNP_001128071filamin-A
4.0±0.7
5
2.45
3
4.0±0.6
6
2.80
3
FlnbNP_001100758filamin-B
4.4±0.3
35
16.15
3
3.4±0.2
30
15.36
3
GapdhNP_058704glyceraldehyde-3-phosphate dehydrogenase
5.0±0.3
7
5.26
3
3.8±0.3
6
4.59
3
Gnai2NP_112297guanine nucleotide-binding protein G(i) subunit alpha-2
5.0±0.6
2
2.40
3
4.1±0.8
2
2.11
3
Golga5NP_001028237Golgin subfamily A member 5
6.2±0.4
4
-
2
5.1±0.4
6
4.25
3
Gstp1NP_036709glutathione S-transferase P
2.4
1
1.84
3
-
-
-
-
Hdac6XP_006256821histone deacetylase 6
5.8
2
-
2
7.0±0.7
2
3.25
3
HdlbpNP_742036vigilin
5.1
1
-
2
4.6±0.5
4
2.59
3
HnrnpkNP_476482heterogeneous nuclear ribonucleoprotein K
4.4±0.3
4
3.11
3
4.2±0.3
3
2.44
3
HnrnpuNP_476480heterogeneous nuclear ribonucleoprotein U
2.8±0.4
4
2.12
3
2.3±0.3
4
2.44
3
Hnrnpul1NP_001101947heterogeneous nuclear ribonucleoprotein U-like protein 1
5.6±0.3
8
6.87
3
4.8±0.3
11
7.41
3
Hsp90aa1NP_786937heat shock protein HSP 90-alpha
3.2±0.6
6
2.45
3
2.4±0.5
6
2.24
3
Hsp90ab1NP_001004082heat shock protein HSP 90-beta
4.4±0.4
10
5.99
3
3.5±0.4
10
4.86
3
Hspa1aNP_114177heat shock 70 kDa protein 1B
4.3±0.5
9
4.42
3
3.3±0.3
12
6.15
3
Hspa2NP_068635heat shock-related 70 kDa protein 2
4.5±0.5
12
5.80
3
4.0±0.5
9
4.20
3
Hspa5NP_03721578 kDa glucose-regulated protein precursor
4.5±0.3
7
5.35
3
4.8±0.8
8
3.20
3
Hspa8NP_077327heat shock cognate 71 kDa protein
4.2±0.1
4
4.21
3
3.5±0.6
5
2.39
3
Hspb1NP_114176heat shock protein beta-1
5.4±0.9
1
1.58
3
5.6±0.4
3
-
2
Hsph1NP_001011901heat shock protein 105 kDa
5.0±0.4
8
5.24
3
4.4±0.8
8
2.97
3
ImmtNP_001030100mitochondrial inner membrane protein
3.9±1.3
3
-
2
4.3±1.4
4
1.67
3
Iqgap1NP_001101959ras GTPase-activating-like protein IQGAP1
3.9±0.2
19
12.10
3
3.3±0.2
23
12.87
3
Itpr1NP_001007236inositol 1,4,5-trisphosphate receptor type 1 isoform 3
5.6±0.5
3
2.17
3
4.4
2
-
2
Kif5bNP_476550kinesin-1 heavy chain
4.5±0.1
4
4.46
3
3.4±0.5
6
2.86
3
Krt1NP_001008802keratin, type II cytoskeletal 1
2.3±0.5
5
1.99
3
-
-
-
-
Krt18NP_446428keratin, type I cytoskeletal 18
4.0±0.5
10
4.72
3
5.2±0.4
9
5.87
3
Krt7NP_001041335keratin, type II cytoskeletal 7
4.7±0.5
6
3.74
3
4.7±1.1
6
2.04
3
Krt8NP_955402keratin, type II cytoskeletal 8
4.1±0.4
8
4.78
3
3.6±0.6
8
3.25
3
Ktn1XP_008768836kinectin isoform X10
5.0±0.5
4
-
2
4.3±0.6
3
1.74
3
LrbaNP_001102025lipopolysaccharide-responsive and beige-like anchor protein
5.8±0.2
49
27.32
3
5.3±0.2
53
27.16
3
Map4NP_001019449microtubule-associated protein 4
5.4±0.6
7
4.06
3
5.9±0.4
8
5.67
3
MtorNP_063971serine/threonine-protein kinase mTOR
6.1±0.2
5
5.34
3
6.2±0.5
4
2.84
3
Myh10NP_113708myosin-10
3.2±0.1
37
25.61
3
3.2±0.2
37
20.40
3
Myh9NP_037326myosin-9
3.4±0.3
37
15.04
3
3.0±0.2
30
15.33
3
Myo1cNP_075580unconventional myosin-Ic
4.2±0.7
10
3.58
3
3.6±0.8
9
2.79
3
Myo1dNP_037115unconventional myosin-Id
5.8±0.3
5
-
2
5.5±1.0
6
2.64
3
Myo1eNP_775124unconventional myosin-Ie
5.3±0.8
7
3.26
3
6.3±0.6
6
3.83
3
Myo6XP_001061392unconventional myosin-VI-like
3.9±0.4
10
5.38
3
4.6±0.3
12
7.54
3
Nae1NP_114461NEDD8-activating enzyme E1 regulatory subunit
6.6±0.4
9
6.45
3
5.7±0.6
10
4.99
3
Pdcd6ipNP_001025081programmed cell death 6-interacting protein
5.5±0.3
4
-
2
4.8±0.4
7
4.52
3
Pdia3NP_059015protein disulfide-isomerase A3 precursor
5.3±0.4
14
8.69
3
5.0±0.5
12
6.14
3
Pdia6NP_001004442protein disulfide-isomerase A6 precursor
5.7±0.1
3
3.27
3
5.5±0.9
5
2.42
3
PhkbNP_001014174phosphorylase b kinase regulatory subunit beta
6.3±0.3
3
-
2
5.6±0.5
3
2.06
3
PkmNP_445749pyruvate kinase PKM
3.0±0.2
16
9.36
3
2.2±0.4
12
3.98
3
Plekha6XP_008767737pleckstrin homology domain-containing family A member 6
7.1±0.5
2
-
2
6.0±1.0
5
2.45
3
Pnpla6XP_008767237neuropathy target esterase isoform X11
5.8±0.4
1
2.42
3
5.5±0.9
2
2.06
3
PplNP_001100446periplakin
4.3±0.4
16
-
2
4.2±0.4
11
5.68
3
Prdx1NP_476455peroxiredoxin-1
4.8±0.4
2
3.56
3
3.1±0.5
3
1.60
3
Ptplad1NP_001100301protein tyrosine phosphatase-like A domain containing 1
5.3±0.9
2
1.52
3
5.1±1.6
3
-
1
PygbNP_037320glycogen phosphorylase, brain form
3.2±0.4
7
3.60
3
2.9±0.3
9
5.39
3
Rpl7aNP_00110786360S ribosomal protein L7a
3.4
1
-
1
2.8±0.4
1
1.78
3
Rpn1NP_037199dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 precursor
4.7±0.4
5
-
2
3.8±0.5
5
2.67
3
Rps2NP_11402640S ribosomal protein S2
5.0±0.5
2
-
2
3.6±0.3
2
2.11
3
Rps8NP_11389440S ribosomal protein S8
4.5±0.3
4
3.11
3
3.4±0.8
3
1.33
3
S100a11NP_001004095protein S100-A11
4.6
1
-
2
5.4±0.7
1
1.74
3
Sec23bNP_001102063protein transport protein Sec23B
4.8±0.4
2
2.23
3
4.5±0.1
2
-
2
Sec24cNP_001102926protein transport protein Sec24C
5.4±1.1
2
1.44
3
4.8±0.4
1
2.17
3
Sec31aNP_148981protein transport protein Sec31A
5.2±0.5
17
7.51
3
5.4±0.4
17
9.97
3
Sept9NP_114025septin-9 isoform 3
4.7±1.0
6
-
2
5.8±0.8
5
2.73
3
Slc14a2NP_062220urea transporter 2 isoform 3
5.7±0.3
10
7.64
3
5.4±0.2
15
14.02
3
Slc25a5NP_476443ADP/ATP translocase 2
4.7±0.4
3
3.36
2
3.5±0.1
4
7.02
3
Sptan1NP_741984spectrin alpha chain, non-erythrocytic 1
4.3±0.3
37
16.92
3
3.3±0.3
39
14.23
3
Sptbn1NP_001013148spectrin beta chain, brain 1
4.8±0.6
7
-
2
3.6±0.9
5
1.78
3
Sptbn2NP_062040spectrin beta chain, non-erythrocytic 2
4.7±0.2
38
20.74
3
3.8±0.2
33
16.91
3
Stat1NP_001029336signal transducer and activator of transcription 1 isoform beta
5.5±0.5
15
7.70
3
4.7±0.5
15
6.49
3
Tgm2NP_062259protein-glutamine gamma-glutamyltransferase 2
5.6±0.4
14
8.84
3
3.3±0.4
9
4.38
3
Tln1NP_001034114talin-1
5.1±0.6
7
3.82
3
3.8±0.5
8
4.20
3
Tor4aNP_001101286torsin family 4, member A
-
-
-
-
3.5±0.8
2
1.33
3
Tpi1NP_075211triosephosphate isomerase
4.3±1.1
3
-
2
3.4±1.0
3
1.54
3
Trap1NP_001034090heat shock protein 75 kDa, mitochondrial precursor
6.6
2
-
2
3.7±0.6
5
2.36
3
Trpv4NP_076460transient receptor potential cation channel subfamily V member 4
6.2±0.8
11
4.91
3
6.7±0.6
12
6.79
3
Tuba1cNP_001011995tubulin alpha-1C chain
5.0±0.3
6
4.84
3
3.8±0.3
4
3.08
3
Tuba4aNP_001007005tubulin alpha-4A chain
5.7±1.0
3
1.51
3
4.6±0.3
5
3.85
3
Tubb2aNP_001102589tubulin beta-2A chain
4.8±1.1
1
1.35
3
4.7±0.3
1
2.44
3
Tubb4aNP_543158tubulin, beta 4
4.1±0.8
1
1.43
3
5.4±0.6
2
-
2
Tubb4bNP_954525tubulin beta-4B chain
6.0±0.8
2
3.27
3
4.8±0.4
3
2.12
3
Tubb5NP_775125tubulin beta-5 chain
6.2±0.6
4
2.76
3
5.8±0.7
4
2.52
3
Uba1NP_001014102ubiquitin-like modifier-activating enzyme 1
5.4±0.5
10
5.98
3
4.3±0.6
12
4.65
3
Uba3NP_476553NEDD8-activating enzyme E1 catalytic subunit
6.1±0.8
6
3.16
3
6.2±0.7
11
5.59
3
Uggt1NP_598280UDP-glucose:glycoprotein glucosyltransferase 1 precursor
4.0±0.9
4
-
2
3.9±0.5
8
4.15
3
Uso1NP_062252general vesicular transport factor p115
5.5±0.6
8
4.41
3
5.1±0.7
7
3.51
3
Usp9xNP_001129365probable ubiquitin carboxyl-terminal hydrolase FAF-X isoform 1
6.1±0.5
18
9.04
3
5.7±0.3
19
11.72
3
VapaNP_113819vesicle-associated membrane protein-associated protein A
4.5
1
-
2
5.2±0.7
2
2.87
3
VarsNP_445744valine--tRNA ligase
6.4±0.3
17
12.48
3
6.0±0.3
20
13.10
3
Vps35NP_001099188maternal embryonic message 3
6.4±0.3
13
10.08
3
4.5±0.2
12
10.15
3
YwhaeNP_11379114-3-3 protein epsilon
5.9±0.8
2
-
2
4.7±0.6
4
2.39
3
YwhaqNP_03718514-3-3 protein theta
3.9±0.4
2
3.42
3
4.5±0.4
4
2.72
3
YwhazNP_03714314-3-3 protein zeta/delta
3.8±0.3
5
3.58
3
4.6±0.6
4
2.27
3

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