Supplemental Material and Methods

for

Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction

Chih-Chien Sung, Brian Poll, Shih-Hua Lin, Adrian R. Murillo-de-Ozores, Chung-Lin Chou, Lihe Chen, Chin-Rang Yang, Min-Hsiu Chen, Yu-Juei Hsu, and Mark A. Knepper

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Supplemental Methods

Supplemental Spreadsheet 1: Intrinsic variability RNA-Seq method (sham control vs.

sham control comparison) in the CCD three hours after UUO

Supplemental Spreadsheet 2: Lists of gene sets for regulatory pathways three hours after UUO

Supplemental Spreadsheet 3: DAVID gene enrichment analysis with KEGG pathways and Gene Ontology Biological Process terms in microdissected CCDs three hours after unilateral ureteral obstruction (UUO)

Supplemental Table 1: Expression of aldosterone targets in microdissected CCDs three hours after UUO

Supplemental Table 2: Secreted proteins increased in both CCD and CTAL three hours after UUO

Supplemental Table 3: Transcript abundance changes in microdissected CCDs of rats at 30 mins after UUO.

Supplemental Figure 1: Primer sequences for RT-qPCR.

Supplemental Figure 2: Female rats with unilateral ureteral obstruction (UUO) display downregulation of AQP2 in ipsilateral kidney.

Supplemental Figure 3: Principal component analysis (PCA) of RNA seq data.

Supplemental Figure 4: Activation of aldosterone-regulated genes after UUO.

Supplemental Figure 5: Time course of the P-bodies after UUO.

Supplemental Figure 6: Decreased AQP2 abundance in culture collecting duct cells (mpkCCD) after lipopolysaccharide (LPS).

Webpage created by Brian Poll and Mark Knepper in the Epithelial Systems Biology Laboratory (Mark Knepper, Chief) at the National Heart, Lung and Blood Institute as part of its Kidney Systems Biology Project. (January 2022)