Cells were grown with media standard for each cell line (see manuscript).
In all cases, cells were grown on permeable supports (Corning Transwell, CLS3450)
and had reached confluence at the time of RNA isolation.
The SMARTer V4 Ultra Low RNA Kit (Clontech) was used to construct cDNA using 10 ng total RNA. 1 ng of the resulting cDNA was
“tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) to generate sequencing libraries for
large-scale sequencing (Illumina HiSeq 3000)
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