Transcriptome of Parvalbumin-Expressing Cells in Mouse Renal Cortex: Effect of Magnesium Intake

(Data from: de Baaij et al. Am J Physiol Renal Physiol. 2013; 305:F1563-73.)

Brief methods:
     The study employed 4 to 6 wk old mice expressing enhanced green fluorescent protein (eGFP) under the parvalbumin (PV) promoter, which fluorescently labels distal convoluted tubule cells. Mice were fed either low [0.02% (wt/wt)] or high [0.48% (wt/wt)] Mg²⁺ diets for 15 days.  PV-eGFP-positive tubules were isolated from the kidneys using COPAS after collagenase/hyaluronidase incubation.  4,000 eGFP-positive fluorescent tubules were collected and RNA was extracted. After amplification, relative RNA abundances were read out using Mouse Whole Genome Gene Expression Microarrays (V2, Agilent Technologies), representing 39,429 Mus musculus 60-mer probes.   For this webpage, transcripts with the 8000 highest signals (after removal of duplicate official gene symbols) are presented.  All data are median-normalized (using the median of the 8000 top values) for ease of comparison to other data sets.   Values above 10 are highlighted in dark green; values between 1 and 10 are highlighted in light green.  P values were determined by a permutation F2-test.  P values less than 0.05 are highlighted in bright yellow.  P values between 0.10 and 0.05 are highlighted in pale yellow.   Questions to: Joost G. J. Hoenderop at Joost.Hoenderop@radboudumc.nl. Website is hosted by: NHLBI Epithelial Systems Biology Laboratory (ESBL).