| Epithelial Systems Biology Laboratory, NHLBI, NIH | |||||
| Identification of Vasopressin-Regulated Transcripts in mpkCCD Cells using SAGE | |||||
| Data from: Robert-Nicoud M, Flahaut M, Elalouf JM, Nicod M, Salinas
M, Bens M, Doucet A, Wincker P, Artiguenave F, Horisberger JD, Vandewalle A,
Rossier BC, Firsov D. Transcriptome of a mouse
kidney cortical collecting duct cell line: effects of aldosterone and
vasopressin. Proc
Natl Acad Sci U S A. 2001;98:2712-6. PMID:
11226305. Methods: Mouse mpkCCDcl4 cells were used, a collecting duct-derived transformed cell line.Cells were treated with 10 nM vasopressin (Vaso4h) for 4 h or with vehicle. The mRNAs of the control and and Vaso4h conditions were prepared in two independent sets of confluent cells grown on filters having identical electrical properties. Poly(A) mRNAs were selected and SAGE libraries were prepared from 5 μg of the corresponding poly(A) mRNAs. 10,000 sequencing reactions were run. Assessment of significant differences between two SAGE libraries was made by Monte Carlo simulation analysis and validated by a Z-test. A P value of 0.05 or less was considered significant. Transcripts significantly increased by vasopressin are labeled as VITs. Transcripts significantly decreased by vasopressin are labeled VRTs. The identifications were re-annotated in 2014 based on current RefSeq data. |
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| Database created by Dmitri Firsov (Lausanne) and Mark Knepper (Bethesda). Email: dmitri.firsov@unil.ch. Database is hosted by the Epithelial Systems Biology Laboratory (Mark Knepper, Chief) of the National Heart, Lung and Blood Institute as part of its Kidney Systems Biology Project. | |||||
| Official Gene Symbol | Designation | SAGE Tag | Counts (control) | Counts (vasopressin) | Annotation |
| Abcb4 | VIT36 | CATTTGTACC | 1 | 18 | multidrug resistance protein 3 |
| Akap1 | VRT5 | TTGGACTTTA | 4 | 1 | A-kinase anchor protein 1, mitochondrial |
| Akap8 | VIT46 | TTCCTGGTAA | 0 | 7 | A-kinase anchor protein 8 |
| Avpi1 | VIT32 | AGACACTGAC | 1 | 28 | arginine vasopressin-induced protein 1 |
| B2m | VIT18 | AGAATAATAA | 6 | 73 | beta-2-microglobulin precursor |
| B2m | VIT25 | ATATGCCAAA | 4 | 41 | beta-2-microglobulin precursor |
| Calm3 | VRT2 | AGGGAAGCAG | 23 | 20 | calmodulin 3 |
| Ccdc73 | VRT11 | CTTCCCCCAC | 3 | 0 | coiled-coil domain-containing protein 73 |
| Cd24 | VRT3 | AGAATAGCCA | 31 | 19 | nectadrin (CD24) |
| Cd9 | VIT27 | CGGGGGTTCA | 10 | 34 | CD9 antigen |
| Cox7c | VIT19 | TGAAAATTGG | 7 | 67 | cytochrome c oxidase subunit 7C, mitochondrial precursor |
| Cstb | VIT11 | TCTCTAAATA | 19 | 131 | cystatin-B |
| Cuzd1 | VRT6 | ACAGCAGAGA | 3 | 0 | CUB and zona pellucida-like domain-containing protein 1 precursor |
| Cycs | VIT22 | ATGAATGGCT | 15 | 52 | cytochrome c, somatic |
| Eef1b | VIT17 | TGATGATGAG | 30 | 81 | Elongation factor 1-beta |
| Erh | VIT15 | TTGTCACTCT | 19 | 94 | enhancer of rudimentary homolog |
| Flna | VRT9 | ACTCTGGCCG | 3 | 0 | filamin-A |
| Foxa1 | VIT38 | ATTTGCAGTT | 0 | 13 | hepatocyte nuclear factor 3-alpha |
| Gm10713 | VIT14 | AGCTGAAGGT | 19 | 98 | predicted pseudogene 10713 |
| Gm2366 | VIT42 | CAGCCAGCCT | 0 | 9 | predicted gene 2366 |
| Gpi1 | VRT4 | AGCTTCATCA | 9 | 6 | glucose-6-phosphate isomerase |
| H2-D1 | VIT12 | TAACACCCTC | 9 | 108 | H-2 class I histocompatibility antigen, D-B alpha chain precursor |
| Hilpda | VIT31 | AGGCCTGCTT | 0 | 28 | hypoxia-inducible lipid droplet-associated protein isoform 1 |
| Hnf3a | VIT38 | ATTTGCAGTT | 0 | 13 | hepatocyte nuclear factor 3-alpha |
| Krt18 | VIT2 | GTGGATGGCA | 58 | 531 | keratin, type I cytoskeletal 18 |
| Krt8 | VIT8 | AGCCCTAATC | 33 | 206 | keratin, type II cytoskeletal 8 |
| Lgals3 | VIT33 | TAAGCCAGAA | 5 | 26 | galectin-3 |
| Mark3 | VRT8 | ATTTGGTGCT | 3 | 0 | MAP/microtubule affinity-regulating kinase 3 isoform 2 |
| Atp6 | VRT1 | AACAAATCTC | 140 | 86 | ATP synthase subunit a (mitochondrial gene) |
| Mzt1 | VRT7 | TCTACAGACA | 4 | 0 | mitotic-spindle organizing protein 1 |
| Nme2 | VIT9 | ATCTTTTCTA | 23 | 167 | nucleoside diphosphate kinase B |
| Oas1a | VIT13 | AACAATAAAC | 4 | 106 | 2'-5'-oligoadenylate synthase 1A |
| Pgp | VIT24 | TAGTGTCTTT | 3 | 42 | phosphoglycolate phosphatase |
| Plac8 | VIT16 | CATTTAATTA | 5 | 90 | placenta-specific gene 8 protein |
| Plet1 | VIT23 | ATCCTGTGGC | 14 | 50 | placenta-expressed transcript 1 protein precursor |
| Ppl | VRT10 | TTAGTAATGG | 3 | 0 | periplakin |
| Ramp3 | VIT48 | TGTCACGATT | 0 | 7 | receptor activity-modifying protein 3 precursor |
| Rhof | VIT39 | GTGGGATACA | 0 | 12 | rho-related GTP-binding protein RhoF precursor |
| Rpl12 | VIT10 | CTGGGTACTG | 24 | 144 | 60S ribosomal protein L12 |
| Rpl26 | VIT3 | GAGAAGATGC | 105 | 447 | 60S ribosomal protein L26 |
| Rpl9 | VIT6 | CTGACCACAT | 85 | 248 | 60S ribosomal protein L9 |
| Rps16 | VIT5 | GGGGTTTACC | 120 | 326 | 40S ribosomal protein S16 |
| Rps23 | VIT7 | ATAAAATTTG | 31 | 223 | 40S ribosomal protein S23 |
| Rps27 | VIT20 | TCCTTCATCC | 6 | 59 | 40S ribosomal protein S27 |
| Rps28 | VIT4 | TGCAACTGTT | 202 | 423 | 40S ribosomal protein S28 |
| Rps29 | VIT1 | ATGCTAGTCT | 450 | 1123 | 40S ribosomal protein S29 |
| Spcs1 | VIT30 | TAATTGTCAT | 4 | 29 | signal peptidase complex subunit 1 |
| Sra1 | VIT28 | ACTTGTTATG | 5 | 31 | steroid receptor RNA activator 1 isoform b |
| Ssr4 | VIT37 | TACTACCTAG | 0 | 14 | translocon-associated protein subunit delta isoform 1 precursor |
| Tacc1 | VIT21 | TCTCTGCTCA | 17 | 53 | transforming acidic coiled-coil-containing protein 1 long isoform |
| Tomm20 | VIT29 | CATTGTCTGT | 6 | 29 | mitochondrial import receptor subunit TOM20 homolog |
| Usp10 | VIT34 | ACAAAGTTGG | 5 | 24 | ubiquitin carboxyl-terminal hydrolase 10 |
| none | VIT26 | TGCAAACTTA | 9 | 38 | |
| none | VIT35 | AGCGCAGCCT | 2 | 19 | |
| none | VIT40 | CAAATCAGGA | 0 | 12 | |
| none | VIT41 | TGAATAGACA | 0 | 10 | |
| none | VIT43 | CGCAAGCGCA | 0 | 9 | |
| none | VIT44 | TTTCGATGTC | 0 | 8 | |
| none | VIT45 | AATGAGGACG | 0 | 7 | |
| none | VIT47 | AACAAGCGTA | 0 | 7 | |