TiPD (Temporal iTRAQ Phosphoproteomic Database)

This database of iTRAQ-labeled renal inner medullary collecting duct (IMCD) phosphopeptides is based on temporal quantitative protein mass spectrometry data from the NHLBI Epithelial Systems Biology Laboratory (ESBL). All data are from IMCD cells freshly purified from rat inner medullas.

Brief methods: IMCD cells were incubated in the presence or absence of a vasopressin analog (dDAVP) for 0.5, 2, 5, and 15 min. Each sample was digested with trypsin, labeled with a different 8-plex iTRAQ reagent, mixed, and analyzed by mass spectrometry with an LTQ Orbitrap Velos. This experiment contains 3 biological replicate time courses.

Reference: Hoffert et al., "Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomics". Mol Cell Proteomics. 2011 Nov 30. [Epub ahead of print]

This website was created by Jason Hoffert, Trairak Pisitkun, Jae Song, Fahad Saeed, and Mark Knepper. Contact us with questions or comments: knep@helix.nih.gov

Current database size: 12,167 phosphopeptides

Database updated: May 5, 2011 (download data in excel)

"Mouse-over" each peptide sequence to view a graph of the relative phosphopeptide abundances [log2(dDAVP/control)] for each time point. Each graph is tagged with a unique identifier that includes the peptide sequence with site(s) of modification (# indicates phosphorylation, @ indicates oxidation, ^ indicates iTRAQ modification of Y), the charge state in parentheses, and finally the version (indicates multiple instances of the same phosphopeptide). Database has been sorted by Gene Symbol.

In order to view temporal clustering information for these peptides, please visit the iTRAQ Cluster Analysis page.