The mTAL Phosphoproteome Database
contains phosphoprotein mass
spectrometry data from native renal medullary thick ascending limb (mTAL)
-enriched tubule suspensions. The database was created by Ruwan Gunaratne, Trairak
Pisitkun, and Mark Knepper of the Epithelial Systems Biology Laboratory, Division
of Intramural Research, NHLBI, NIH. Bethesda.Please cite:Gunaratne R,
Braucht DW, Rinschen MM, Chou CL, Hoffert JD, Pisitkun T, Knepper MA.
Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent
signaling pathways in native renal thick ascending limb cells. Proc Natl Acad Sci U S A.
2010;107:15653-8.PMID: 20713729. Contact us for questions or comments.
Experimental procedures:
Large-scale quantitative phosphoproteomic profiling was performed on paired
vehicle- and hormone-treated mTAL-enriched suspensions. Hormone treatment utilized a mixture of hormones that increase cAMP levels (vasopressin, glucagon, parathyroid hormone, and calcitonin) through GPCRs. The samples were
analyzed on an LTQ-Orbitrap mass spectrometer and the resulting MS spectra
were searched using three search algorithms: SEQUEST, InsPecT, and OMSSA. In
all cases, a target-decoy analysis approach was undertaken to limit the false
discovery rate to < 2%. Phosphorylation site assignment was carried out
using the following scoring algorithms: Ascore and PhosphoScore for SEQUEST
data, and Phosphate Localization Score for InsPecT data. In order to assign a
phosphorylation site correctly, at least one score had to pass the given
threshold (Ascore = 19, PhosphoScore was Passed or OneChoice, PLS score =
8). Final assignment of phosphorylation sites was performed using the
in-house programs, NHLBI Promatch and Phosphosite. Quantification of
phosphopeptide abundance (area under the curve of the reconstructed ion
chromatogram elution profile) was achieved using QUOIL, an in-house software
program designed for quantification of label-free peptides by LC-MS (NHLBI
Proteomics Core Facility). As a result, 654 unique phosphopeptides
corresponding to 374 unique phosphoproteins were identified.
FERM, RhoGEF and pleckstrin
domain-containing protein 1
NP_001100757
LGAPENSGIS*TLER
S23
0.13
9.28
-0.06
3.12
3.08
Farp1
FERM, RhoGEF and pleckstrin
domain-containing protein 1
NP_001100757
LGAPENSGIST*LER
T24
0.13
9.27
-0.07
3.11
3.08
Farp1
FERM, RhoGEF and pleckstrin
domain-containing protein 1
NP_001100757
SPDEATAADQES*EDDLSASR
S893
0.49
n/a
n/a
Fcho2
Fcho2 isoform 2
XP_219503
LSGINEIPRPFS*PPITSNTS*PPPTAPLAR
S533, S541^
-0.23
n/a
n/a
Fkbp15
FK506 binding protein 15
XP_342847
RLS*LTPDPEKK
S1094^
-0.35
7.24
-0.16
2.24
2.50
Fkbp2
peptidyl-prolyl cis-trans
isomerase FKBP2
NP_001127900
VDHCPIKS*R
S44
-2.66
n/a
n/a
Fkbp8
peptidyl-prolyl cis-trans
isomerase FKBP8
NP_001032257
LEPS*NKTIHAELSKLVK
S329
-1.45
13.89
n/a
n/a
Flna
filamin-A
NP_001128071
RAPS*VANVGSHCDLSLK
S2144
0.16
0.59
0.05
0.27
0.16
Fnbp1l
formin-binding protein
1-like
NP_001034698
RHS*SDINHLVTQGR
S488
1.30
2.66
1.29
1.75
0.45
Foxp1
forkhead box protein P1
NP_001029303
ERLQAMMT*HLHVKSTEPKAAPQPLNLVS*S*VTLSK
T401, S421, S422^
-0.77
2.67
0.12
0.67
1.03
G3bp1
GTPase activating protein
(SH3 domain) binding protein 1
NP_598249
SAS*PAPADVAPAQEDLR
S231
0.14
0.65
-0.29
0.17
0.27
G3bp1
GTPase activating protein
(SH3 domain) binding protein 1
NP_598249
YQDEVFGGFVTEPQEES*EEEVEEPEER
S149 ^
-0.31
n/a
n/a
Gbf1
golgi-specific brefeldin
A-resistance factor 1
XP_219953
ADAPDAGAQSDS*ELPSYHQNDVSLDR
S1301^
-0.57
-0.21
n/a
n/a
Gck
glucokinase
NP_036697
VMLVKVGEGEAGQWS*VKTK
S100^
-0.59
-0.37
-0.75
-0.57
0.11
*
Gck
glucokinase
NP_036697
VMLVKVGEGEAGQWSVKT*K
T103^
-0.60
-0.35
-0.75
-0.57
0.12
*
Gng12
guanine nucleotide binding
protein (G protein), gamma 12
XP_001067408
ASADLMS*YCEEHARSDPLLMGIPTSENPFK
S41^
0.33
0.82
0.01
0.38
0.24
Gng12
guanine nucleotide binding
protein (G protein), gamma 12
XP_001067408
S*DPLLMGIPTSENPFK
S49
0.91
0.48
0.51
0.63
0.14
*&
Golga5
golgin subfamily A member 5
NP_001028237
KKS*EPDDELLFDFLNSSQK
S116
0.79
3.42
-0.47
1.25
1.15
Golgb1
Golgin subfamily B member 1
NP_620240
DQHGTLEAGPLNDTGMELNS*PQPDGVDK
S614
0.57
0.73
0.63
0.64
0.05
*&
Grlf1
glucocorticoid receptor
DNA-binding factor 1
XP_001053554
KVS*AVSKPVLYR
S1046
0.24
-0.10
0.51
0.22
0.18
Gsk3a
glycogen synthase kinase-3
alpha
NP_059040
GEPNVS*YICSR
S278^
-5.04
-1.46
1.67
-1.61
1.94
Gsk3a
glycogen synthase kinase-3
alpha
NP_059040
GEPNVSY*ICSR
Y279
-0.33
0.99
-0.30
0.12
0.44
H3f3b
histone H3.3
NP_446437
FQS*AAIGALQEASEAYLVGLFEDTNLCAIHAK
S87^
-2.61
7.23
-0.45
1.39
2.99
Hdac2
histone deacetylase 2
NP_445899
MLPHAPGVQMQAIPEDAVHEDS*GDEDGEDPDKR
S394
-0.36
12.37
-0.15
3.95
4.21
Hdgf
hepatoma-derived growth
factor
NP_446159
AGDMLEDS*PKRPK
S165
-0.75
0.21
-0.28
-0.27
0.28
Hdgf
hepatoma-derived growth
factor
NP_446159
S*CAEEPEVEPEAHEGDGDK
S107
0.99
n/a
n/a
Hist1h1d
histone H1.2
NP_579819
KAS*GPPVSELITK
S36
0.00
-0.08
-0.05
-0.04
0.02
Hist1h1d
histone H1.2
NP_579819
RKAS*GPPVSELITK
S36
-1.05
0.57
1.53
0.35
0.75
Hist1h1d
histone H1.2
NP_579819
S*GPPVSELITK
S36^
-0.07
-0.46
1.32
0.26
0.54
Hist1h2ail
histone H3.1
NP_001013074
FQSSAVMALQEACEAY*LVGLFEDTNLCAIHAK
Y100
-0.63
5.31
-1.74
0.98
2.19
Hist1h4b
histone H4
NP_073177
RIS*GLIYEETR
S48
-0.35
-0.46
-0.23
-0.34
0.07
*
Hmgn1
high-mobility group
nucleosome binding domain 1
NP_001013202
QAEVADQQTTDLPAENGETENQSPAS*EEEK
S87^
0.96
n/a
n/a
Hn1
hematological and
neurological expressed 1 protein
NP_001005876
S*NSSEASSGDFLDLK
S80^
0.88
0.38
0.01
0.42
0.25
Hnf1a
hepatocyte nuclear factor
1-alpha
NP_036801
MVS*KLS*QLQTELLAALLES*GLS*K
S3^, S6^, S19^, S22^
-0.25
4.85
0.11
1.57
1.64
Hnrnpa1
heterogeneous nuclear
ribonucleoprotein A1
NP_058944
SES*PKEPEQLR
S6
0.47
0.50
n/a
n/a
Hnrnpa1
heterogeneous nuclear
ribonucleoprotein A1
NP_058944
SES*PKEPEQLRK
S6
-1.67
-0.19
n/a
n/a
Hnrnpa3
heterogeneous nuclear
ribonucleoprotein A3 isoform a
NP_937765
SSGS*PYGGGYGSGGGSGGYGSR
S359
-0.14
-0.99
-0.37
-0.50
0.25
Hnrnpa3
heterogeneous nuclear
ribonucleoprotein A3 isoform a
NP_937765
SSGSPY*GGGYGSGGGSGGYGSR
Y361
0.05
-0.25
n/a
n/a
Hnrnpa3
heterogeneous nuclear
ribonucleoprotein A3 isoform a
NP_937765
SSGSPYGGGY*GSGGGSGGYGSR
Y365
0.92
-0.53
-2.54
-0.72
1.00
Hnrnpc
heterogeneous nuclear
ribonucleoprotein C
NP_001020804
GDDQLELKDDEKEPEEGEDDRDS*ANGEDDS
S291
1.57
n/a
n/a
Hnrnpk
heterogeneous nuclear
ribonucleoprotein K
NP_476482
GS*YGDLGGPIITTQVTIPK
S379
2.84
0.55
0.27
1.22
0.81
Hnrnpk
heterogeneous nuclear
ribonucleoprotein K
NP_476482
IIPTLEEGLQLPS*PTATSQLPLESDAVECLNYQHYK
S116
-0.05
-0.05
n/a
n/a
Hnrnpu
heterogeneous nuclear
ribonucleoprotein U
NP_476480
AKS*PQPPVEEEDEHFDDTVVCLDTYNCDLHFK
S245
-0.05
-1.16
0.19
-0.34
0.42
Hnrph1
heterogeneous nuclear
ribonucleoprotein H
NP_543172
HTGPNS*PDTANDGFVR
S27
0.10
1.70
-0.27
0.51
0.60
Hnrph1
heterogeneous nuclear
ribonucleoprotein H
NP_543172
HTGPNSPDT*ANDGFVR
T30
0.03
-0.97
-0.36
-0.43
0.29
Hsd11b2
corticosteroid
11-beta-dehydrogenase isozyme 2
NP_058777
ALRPGQPGPVHDTTQDPNPS*PTVSAL
S394
-0.35
2.41
-0.68
0.46
0.98
Hsp90aa1
heat shock protein HSP
90-alpha
NP_786937
ESDDKPEIEDVGS*DEEEEEK
S263
2.32
n/a
n/a
Hsp90aa1
heat shock protein HSP
90-alpha
NP_786937
ESDDKPEIEDVGS*DEEEEEKK
S263
0.78
n/a
n/a
Hsp90aa1
heat shock protein HSP
90-alpha
NP_786937
ESDDKPEIEDVGS*DEEEEEKKDGDK
S263
-0.56
n/a
n/a
Hsp90ab1
heat shock protein HSP
90-beta
NP_001004082
IEDVGS*DEEDDSGK
S255
1.77
n/a
n/a
Hsp90ab1
heat shock protein HSP
90-beta
NP_001004082
IEDVGS*DEEDDSGKDK
S255
0.28
n/a
n/a
Hsp90ab1
heat shock protein HSP
90-beta
NP_001004082
IEDVGS*DEEDDSGKDKK
S255
-0.80
n/a
n/a
Hspa4
heat shock 70 kDa protein 4
NP_705893
MQVDQEEPHTEEQQPQT*PAENK
T538
1.78
0.84
0.28
0.97
0.44
Hspa9
stress-70 protein,
mitochondrial
NP_001094128
EAAEKAKCELSSSVQT*DINLPYLTMDASGPK
T325^
-0.19
0.85
-0.66
0.00
0.45
Hspb1
heat shock protein beta-1
NP_114176
QLS*SGVSEIR
S85
-0.19
4.51
-0.21
1.37
1.57
Hspc159
galectin-related protein
NP_001128202
LDDGHLNNSLGS*PVQADVYFPR
S25
0.41
-0.14
n/a
n/a
Htatsf1
HIV-1 Tat specific factor 1
NP_001101729
VFDDS*DEKEDEEDTDVR
S698
0.80
n/a
n/a
Inadl
InaD-like (Drosophila)
isoform 2
XP_001055452
KTSLSAS*PFEQPSSR
S459^
-0.50
2.51
-0.90
0.37
1.08
Jph1
junctophilin-1
NP_001100100
SESKSS*IS*S*KR
S231^, S233^, S234^
n/a
n/a
Kif26a
kinesin-like protein KIF26A
NP_001163819
LLPS*PAPPPPR
S942
3.02
1.37
2.89
2.42
0.53
*&
Ktn1
kinectin 1
XP_341306
EIQNGTLHES*DSEHVPQDFK
S75
-0.50
4.65
-0.14
1.34
1.66
Ktn1
kinectin 1
XP_341306
EIQNGTLHESDS*EHVPQDFK
S77^
-0.42
4.60
-0.13
1.35
1.63
Lad1
ladinin
NP_001101412
ALPGKS*PPSSAEQSAPDPPTK
S320^
0.54
-0.19
-0.46
-0.04
0.30
Lad1
ladinin
NP_001101412
GRPEETAAQQKS*PVSEK
S181
4.26
-0.13
n/a
n/a
Lad1
ladinin
NP_001101412
LPS*VEEAEVSKPSPPAS*K
S62, S76^
1.32
1.99
-0.02
1.10
0.59
Lad1
ladinin
NP_001101412
LPS*VEEAEVSKPSPPAS*KDEGEEFQAILR
S62^, S76^
-1.41
1.27
2.16
0.68
1.07
Lad1
ladinin
NP_001101412
LPS*VEEAEVSKPSPPASK
S62
0.77
0.17
0.10
0.34
0.21
Lad1
ladinin
NP_001101412
LPS*VEEAEVSKPSPPASKDEGEEFQAILR
S62
-1.91
-0.09
n/a
n/a
Lad1
ladinin
NP_001101412
NLS*STTEDESPK
S38
0.97
n/a
n/a
Lad1
ladinin
NP_001101412
S*VEEAEVSKPSPPASK
S62
0.34
-0.39
-0.39
-0.15
0.24
Lad1
ladinin
NP_001101412
S*VEEAEVSKPSPPASKDEGEEFQAILR
S62
-4.68
-1.43
n/a
n/a
Larp1
la related protein
XP_220446
ESPRPPAAAEAPAGS*DGEDGGR
S132
1.89
-0.16
n/a
n/a
Larp7
la-related protein 7
NP_001037755
ELVVDSSS*SGVSKATK
S166
-0.04
-1.03
-0.80
-0.62
0.30
Lcp1
plastin-2
NP_001012044
GS*VSDEEMMELR
S5^
0.00
0.33
n/a
n/a
Lig4
ligase IV, DNA,
ATP-dependent
NP_001099565
AAEIVPS*DMYKTGSTLRFPRIEK
S567^
-0.84
3.11
-0.29
0.66
1.24
Lima1
LIM domain and actin
binding 1
XP_217039
FGS*RPEAVTQCR
S132
2.70
3.84
2.10
2.88
0.51
*&
Lima1
LIM domain and actin
binding 1
XP_217039
GENEETLGRPAQPPSAGET*PHSPGVEDAPIAK
T485
-0.30
-0.10
n/a
n/a
Lima1
LIM domain and actin
binding 1
XP_217039
GENEETLGRPAQPPSAGETPHS*PGVEDAPIAK
S488
-0.30
2.21
-0.15
0.59
0.81
Lima1
LIM domain and actin
binding 1
XP_217039
GWSEPEPEQSEEFGGGTVTQT*ESPRPSR
T634^
-0.36
-2.12
n/a
n/a
Lima1
LIM domain and actin
binding 1
XP_217039
KGWSEPEPEQSEEFGGGTVTQTESPRPS*R
S640
-0.99
n/a
n/a
Lima1
LIM domain and actin
binding 1
XP_217039
PAQPPSAGETPHS*PGVEDAPIAK
S488
1.26
10.48
-0.42
3.77
3.39
Lima1
LIM domain and actin
binding 1
XP_217039
RLS*ENSCSLDDLEIGAGHLSSSAFNSEK
S225
0.80
-0.65
2.07
0.74
0.79
Lima1
LIM domain and actin
binding 1
XP_217039
RLSENSCS*LDDLEIGAGHLSSSAFNSEK
S230^
0.79
-0.53
1.12
0.46
0.50
Lima1
LIM domain and actin
binding 1
XP_217039
S*PLEPESPGWPGFGDTTTAK
S697
0.33
1.11
1.95
1.13
0.47
Lima1
LIM domain and actin
binding 1
XP_217039
SEAQQPIYTKPLS*PDAR
S360
-0.04
2.61
-0.27
0.76
0.92
Lima1
LIM domain and actin
binding 1
XP_217039
TSSVKS*PKPLSPSLR
S603^
-3.45
-0.05
-0.75
-1.42
1.04
Llgl2
lethal(2) giant larvae
protein homolog 2
NP_001121021
SQS*DGAETKPGPVMEHALLSDAWVLK
S970^
0.36
-0.30
-2.70
-0.88
0.93
Lmna
lamin-A isoform C2
NP_001002016
LRLS*PSPTSQR
S389
-0.82
-0.99
-0.41
-0.74
0.17
*&
Lmo7
LIM domain only protein 7
NP_001001515
EVSRS*PDQFSDMR
S1061
-3.47
0.29
-0.21
-1.13
1.18
Lmo7
LIM domain only protein 7
NP_001001515
S*KSLSDVSAEDVQSLR
S650
0.14
-1.80
-0.92
-0.86
0.56
Lmo7
LIM domain only protein 7
NP_001001515
S*LSDVSAEDVQSLR
S652
0.15
3.51
-0.02
1.21
1.15
Lmo7
LIM domain only protein 7
NP_001001515
SKS*LSDVSAEDVQSLR
S652^
0.17
-0.40
n/a
n/a
Lmo7
LIM domain only protein 7
NP_001001515
SLS*DVSAEDVQSLR
S654
0.15
3.47
-0.02
1.20
1.14
Lmo7
LIM domain only protein 7
NP_001001515
RGES*LDNLDSQRPGSWR
S1563
-0.23
0.16
0.09
0.01
0.12
LOC360570
myosin 18a
NP_001165608
RFS*FSQR
S140
-0.45
0.92
-0.10
0.12
0.41
LOC360570
myosin 18a
NP_001165608
SLAPDLS*DDEHDPVDSISRPR
S2020
-0.28
0.70
-0.07
0.12
0.30
LOC360713
pleckstrin homology-like
domain, family B, member 2-like
XP_001066275
KGS*LQDVDIAGFGSLGHSASFLAPR
S510
1.20
1.51
0.93
1.22
0.17
*&
LOC360713
pleckstrin homology-like
domain, family B, member 2-like
XP_001066275
RLS*AGTTVADVQK
S465
0.30
7.77
-0.11
2.66
2.56
LOC361100
topoisomerase II beta
NP_001094328
KTSFDQDS*DVDIFPSDFTSEPPALPR
S1570
0.02
-0.65
n/a
n/a
LOC361346
hypothetical protein
LOC361346 precursor
NP_001017462
SKKY*IGRQDSLDIK
Y155^
-1.03
2.36
-0.58
0.25
1.06
LOC366669
similar to Nesprin-2
(Nuclear envelope spectrin repeat protein 2) (Syne-2) (Synaptic nuclear
envelope protein 2) (Nucleus and actin connecting element protein) (NUANCE
protein)
XP_001080795
LTSHTPGLDDEKEAS*ENETDIEDPR
S6383^
-1.50
-3.33
n/a
n/a
LOC366669
similar to Nesprin-2
(Nuclear envelope spectrin repeat protein 2) (Syne-2) (Synaptic nuclear
envelope protein 2) (Nucleus and actin connecting element protein) (NUANCE
protein)
XP_001080795
SISEGHPWHVPDS*PSHSK
S6483^
-0.37
-1.46
2.27
0.15
1.11
LOC366669
similar to Nesprin-2
(Nuclear envelope spectrin repeat protein 2) (Syne-2) (Synaptic nuclear
envelope protein 2) (Nucleus and actin connecting element protein) (NUANCE
protein)
XP_001080795
TEEGDT*PPIEAALLDFPR
T4207
0.91
-1.90
n/a
n/a
LOC680758
rCG41835-like
XP_001058748
DGIGHT*PVRERPEE
T120
-2.63
-3.61
1.16
-1.69
1.46
LOC681101
hypothetical protein
XP_001060309
WPPGPAAGS*APSGDGR
S125
-0.59
0.06
-0.29
-0.27
0.19
LOC684097
sorting nexin-3
NP_001037748
RYS*DFEWLR
S72
3.61
2.82
3.99
3.47
0.34
*&
LOC684233
spenito-like
XP_001068152
SLS*PGGAALGYR
S293
-0.10
2.07
0.21
0.72
0.68
LOC685179
SWI/SNF-related
matrix-associated actin-dependent regulator of chromatin c2 isoform 2