Supplementary Material

for

Signaling Processes that Initiate Lithium-Induced Polyuria

Chih-Chien Sung^{1, 2}*, Lihe Chen¹*, Kavee Limbutara¹*, Hyun Jun Jung¹, Gabrielle G. Gilmer¹, Chin-Rang Yang¹, Sookkasem Khositseth^1,3, Shih-Hua Lin², Chung-Lin Chou¹, and Mark A. Knepper¹

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Supplementary Dataset 1: Intrinsic variability RNA-Seq method (control vs. control comparison) in the CCD

Supplementary Dataset 2: Full list of transcripts from CCD and summary of observed changes

Supplementary Dataset 3: Full list of transcripts from cTAL and summary of observed changes

Supplementary Dataset 4: Chi-square analysis for overrepresented signaling pathways

Supplementary Dataset 5: Time course of transcript changes after adding LiCl to diet

Supplementary Dataset 6: Proteomic analysis

Supplementary Figure 1: RNA-Seq Quality Control

Supplementary Figure 2: How cell counting was performed

Supplementary Figure 3: Primer sequences for RT-qPCR

Webpage created by Gabrielle Gilmer and Mark Knepper in the Epithelial Systems Biology Laboratory (Mark Knepper, Chief) at the National Heart, Lung and Blood Institute as part of its Kidney Systems Biology Project. (July 2018)